ABSTRACT The susceptibilities of Salmonella typhi and other pathogens to antibiotics and hot aqueous extract of Hibiscus sabdariffa were investigated using agar diffusion and agar well diffusion methods respectively. Salmonella typhi was sensitive to ampicillin, cetriaxone, ciprofloxacin, gentamycin of ofloxacin and perfloxacin. Nitrofurantoin,... Continue Reading
ABSTRACT Some locally available fruits (garden egg, orange, banana, guava, avocado, pawpaw and pineapple).sold in Oye Emene Enugu, Enugu State was examined for parasitological contamination. A total of 87 samples were examined using sedimentation and concentration methods. 11(12.6%) of the 87 fruits were positive for intestinal parasites... Continue Reading
ABSTRACT Safety of food is a basic requirement of food quality. A total of 25 street food samples (Jollof rice, egwusi soup, ugu, water leaf and green), were randomly purchased from five different vendors in Ogbete main market Enugu. The samples were transported in ice to the laboratory. The samples were bacteriologically analyzed using pour plate... Continue Reading
ABSTRACT A total of 50 blood (30 pregnant and 20 HIV) samples were obtained from pregnant women and PLWHA attending clinic at UNTH Ituku-ozalla. Samples were screened for hepatitis c viral infection using the rapid one step hepatitis C virus test strip. Two (6.66%) pregnant women were positive and four (20%) HIV patients were positive for... Continue Reading
ABSTRACT Effect of different carbon sources on the growth of antimicrobial producing Bacillus species in ogiri-egusi sold in Ogbeta, Orie, Artisian, Kenyatta, Enugu State were evaluated using standard microbiological and analytical methods. 12 bacteria were isolated and identified such as Bacillus subtilis,(33.33), Bacillus pumulis (41.66),... Continue Reading
ABSTRACT Comparative examination of three main sources of water supply in Independence layout Enugu was carried out with a view to determine their levels of contamination of bacteria. The sources of water examined are tap water, well water and stream water. The pour plate method was used to examine the water samples. Bacteria isolated from the... Continue Reading
ABSTRACT A major challenge for achieving successful mosquito control is overcoming insecticide resistance. Bacillus thuringiensis which is one of the most effective biolarvacide for control of species of mosquitoes and monitoring of larval susceptibility is essential to avoid resistance development. Mosquito larvacidal activity of Bacillus... Continue Reading
ABSTRACT Antibacterial activity of honey obtained from two different locations in Enugu State (Nsukka & Ugwuaji) Nigeria on Staphylococcus aureus, Escherichia coli and Streptococcus pyogens isolated from wound was studied. Agar well diffusion method was used to determine the antibacterial activity of the honey on the test microorganisms. The... Continue Reading
ABSTRACT Fresh healthy carrot promote good health but harbour a wide range of contaminants. To assess the microbial quality of carrots sold in Ibadan, a total of fifteen samples were purchased from five vendors. Samples were analyzed to study the density of microorganisms by standard plate count. ... Continue Reading
ABSTRACT Antibacterial activity of honey obtained from two different locations in Enugu State (Nsukka & Ugwuaji) Nigeria on Staphylococcus aureus, Escherichia coli and Streptococcus pyogens isolated from wound was studied. Agar well diffusion method was used to determine the antibacterial activity of the honey on the test microorganisms. The... Continue Reading
ABSTRACT A comparative study was done on the physico-chemical and antioxidant properties of some culinary herbs and local spices. Piper Guiness (Uziza), Xylopia aethopica (Uda), Monodora Myristica (Ehuru) and Trtraleura Tetraopera (Oshsho). The proximate analysis of these spices were done to determine their moisture content, ash content,... Continue Reading
ABSTRACT Thirty ripe mango fruits were pluked under sterile conditions from different geographical locations in Enugu metropolis. The mango fruits were exposed to open environment for 5 – 7 days to allow decay. The decaying mango fruits were washed with sterile water to extract the microorganisms. Using the streak plate method, the... Continue Reading
ABSTRACT The antibacterial activity of sweet orange(citrus smensin) on staphylococcus aureus and Escherichia coli isolated from would infection was studied. A total number of 100 wound sample were collected form different individual from different age range within 5-8years and examined,. The test organisms S auras and E coli were cultured... Continue Reading
ABSTRACT Microbial examination of spoilt Avocado fruits was carried out to obtained the causal organism. A total of 60 avocado from Ogbete Main market and New market Enugu were examined by culturing on nutrient agar and sabourand dextrose agar (SM), for bacteria and fungi causing post – harvest spoilage on the fruits; six bacterial species and... Continue Reading
ABSTRACT The isolation, identification and characterization of bacteria from organic waste were carried out. Sawdust waste (plant organic waste source) and abattoir waste water (animal organic waste source) samples were collected. The media used were nutrient agar for total aerobic plate count, McConkey agar for coliform count and cellulolytic... Continue Reading
ABSTRACT Microorganisms are essential players in the very existence of all living thing. They play a very important part in the production and biodegradation of organic food substances in which Manihot esculenta is an important player. This project evaluated the mycoflora and bacterial flora of steep cassava with the view of identifying those... Continue Reading
ABSTRACT . Microbial quality of commercially prepared yoghurt sold in umuahia was evaluated. Ten(10) different brands of Yoghurt was purchased randomly from hawkers and beverage stores in Umuahia urban and analyzed in the laboratory. Six (6) of the samples where registered by NAFDAC [ Sample A, B, C, D, E and F,] while four(4) others were not... Continue Reading
ABSTRACT The antibiotic susceptibility pattern of six Gram negative pathogenic bacteria; Escherichia coli, Pseudomonas, Klebsiella, Salmonella, Shigella and Enterococcus was evaluated. The test organisms were subjected to antibiotic susceptibility test using the Kirby Bauer method. A multi-disc containing eight (8) antibiotics; Ceftrazidime... Continue Reading
ABSTRACT Microbial analysis was carried out on six samples of toasted bread bought from three different kiosks in Unilorin walkway. The microbial population of each sample was determined and related to a variety of physiochemical parameters that could have contributed to their population. These parameters included the pH which varied from 5.37 –... Continue Reading
ABSTRACT The strategy of utilizing mechanochemical synthesis to obtain metal-organic frameworks with high surface area and nanodisperse particles has been espoused. Transition metal ions have been shown to form complexes with organic moieties such as benzyl-N,N-dimethyldithiocarbamate. It has been established that iron in its oxidised state (Fe3+)... Continue Reading
ABSTRACT This study reviewed the importance of hand washing and health benefits derived from the practice and also highlighted the consequences of not observing strict hand washing practice. Swabs from primary school pupils showed cfu mean total viable count of 1.60 x 104, 1.50 x 104, 1.60 x 104 and 1.7 x 104 across the four schools. Salmonella... Continue Reading
ABSTRACT Milk has been known to be highly nutritive ad thus a good medium for bacteria growth and vehicles for transmission of food borne infection. A total of 16 samples of different brands of bagged milk obtained from some selected markets within Kaduna metropolis were analyzed for the occurrence of bacteria using pour plate method on selective... Continue Reading
1.0 INTRODUCTION 1.1 BACKGROUND OF STUDY Schistosomiasis is a chronic human disease caused by parasitic flatworms called schistosomes or blood flukes. The schistosomes live i1n the blood vessels associated with the intestine and urinary bladd1er where they reproduce sexually. Schistosomiasis is one of the most wide spread parasitic disease that... Continue Reading
ABSTRACT This study was carried out to investigate the nutritional value and the antibacterial activity of bitter kola, (Garcinia kola). The result of the proximate analysis shows that bitter kola contains 51.95% moisture, 5.55% Ash, 9.75% crude fibers, 4.65% crude fat, 7.35% crude protein and 20.85% carbohydrate. The anti-bacterial activity shows... Continue Reading
INTRODUCTION 1.1 Background of study Juice is a liquid that is naturally contained in fruit and vegetables. It can refer to liquid that are flavoured with these or other biological food resources such as a beverage or used as an ingredient or flavouring in food, (Lewis, 2002), fruit juice is produce by squeezing or crushing fruit. Many... Continue Reading
ABSTRACT Gastro Intestinal Helminthiasis was investigated among primary school children in Ihengwu village, Oko between February and March 2015. Sixty samples of freshly collected faeces from sixty randomly selected primary school children between the age of four and ten years from two selected primary schools; Gaius Benton and Pope John primary... Continue Reading
ABSTRACT This study was conducted to examine the fungi present in spoilt onion bulb. Five different sample of spoilt onion bulb were blended together and suspended in sterilized distilled water which was used to inoculate Sabouraud dextrose agar (SDA). Four different fungi were isolated from the sample and the morphological characteristics shows... Continue Reading
Abstract The ectoparasitic infestation of free range domestic fowls were surveyed between August and November, 2014. A total of 105 ectoparasites belonging to four species of two genera were collected. These were made up of three lice and one ixodid tick. The percentage occurrence of these parasites are: Menacanthus stramineus (50.5%),... Continue Reading
ABSTRACT The microbiological assessment of Garri, sold in two different markets in Benin City metropolis, was carried out using the pour plate technique. The mean total count ranged from 15.0 to 29.4 × 104 cfu/g in Oba market and Santana market respectively. A total of six organisms were identified in Santana Market samples while a... Continue Reading
ABSTRACT 40 nasal samples were collected from apparently healthy males students at Eguare Secondary School, Ekpoma, whose ages fell within the ranges of 15-20 years were selected and screened for Staphylococcus aureus and Candida albicans using Mannitol Salt Agar and Sabouraud dextrose agar respectively. A total of 15 (37.5%)... Continue Reading
ABSTRACT The results obtained from this research work shows that land snails sold in Ekpoma market contains some pathogenic microorganisms. A total of fifty samples were bought from 10 different local sellers (five snail samples each) in Ekpoma old market and the new market. The total viable count of bacteria ranged from 2.2 x 108 cfu/g - 8.2 x... Continue Reading
ABSTRACT Nunu is a spontaneously fermented yoghurt-like milk product consumed as a staple food commodity invirtually all parts of the Country. Its production and consumption gives way to much food security and economic benefits to the rural people. However, the process characteristics result in products which are not appealing to many people, have... Continue Reading
ABSTRACT A study was undertaken to determine typical concentration of airborne bacteria and fungi (microflora) in Enugu State University of Science and Technology (ESUT) in the tropical rainforest environment of Nigeria. Aerial sampling was conducted at various faculties and departments each day. The air samples were collected thrice daily.... Continue Reading
CHAPTER ONE 1.1 INTRODUCTION Plants have provided a source of inspiration for novel drug compounds, as plant derived medicines have made large contributions to human health and wellbeing. Plant extracts have been used for a wide variety of purposes for many thousands of years (Jones, 1996). The antimicrobial activity of plant oils and extracts has... Continue Reading
Abstract Soaps and other cleansing agents have been around for quite a long time, a medical document from about 1500 B.C. shows that Egyptians combined animal and vegetable oils with alkaline salts to form a soap-like material used for treating skin diseases, as well as for washing and Scrubbing body or hands. The objective of this study is to... Continue Reading
ABSTRACT The antibacterial activity of three types of medicated soap on Staphylococcus aureus isolated from wound infections was conducted. Fourteen individuals with wound infections were sampled. Swab sticks were used to collect specimens from wound infections; each swab stick was streaked separately over plates of nutrient and mannitol agar and... Continue Reading
The introduction Methodology Materials needed Characteristics of bacillus that can produce amylase isolated from Palm kernel waste water in the production of local palm oil Characteristics of bacillus specie that have the potential to bioflocculate isolated from Palm kernel waste water in the production of local palm oil Aim and objectives of... Continue Reading
ABSTRACT The present study is to isolate fungi from rhizosphere of Moringa oleifera and screen them for the production of some very important enzymes such as amylase and protease. Twenty fungi were isolated from the soil sample from five different farms using pour plate method. The isolates were then identified based on their colonial morphology... Continue Reading
1.0 INTRODUCTION AND LITERATURE REVIEW Ear wax is normally produced in the ear canal. It is made up of the secretions of specialized sets of glands located in the ear canal. Sebaceous glands secrete sebum. Sebum is mostly a combination of fatty acids. Modified apocrine sweat glands release a secretion that combines with the sebum to form... Continue Reading
ABSTRACT Antimicrobial activity of ethanolic extract of Mangifera indica peel was tested on some pathogenic organisms. The extract was tested for activity against, Baciollus subtilis, Staphylococcus aureus, Escherichia coli, Salmonela typhii, Pseudomonas notatum and Aspergillus fumigates. The extract exhibited activity against the organisms in... Continue Reading
Mutagenesis Lab Procedure 1. Clean your hands and work area. • Clean your hands with hand sanitizer • Put all the food away 2. Acquire a Petri dish from your instructor and label it using a waterproof marker. • Write your name in small letters around the outside edge of the bottom of the dish. • Draw lines on the bottom of the dish to... Continue Reading
ABSTRACT Colocynthis citrullus (Egusi) are seeds of a creeping annual and intercropping plant made use in traditional farming practices while Irvingia gabonensis (Ogbono) are seeds of small mango-like fruit found in West Africa. The seeds when ground into paste are highly mucilaginous and are used in thickening soups. They also add to the... Continue Reading
ABSTRACT Tinea pedis, a fungal infection of the feet, especially within the toe webs and soles was studied using 40 soldiers (20men and 20women) as a case study. This infection is related to footwear having been considered to be a new dermatophytoses. The soldiers have the toe webs and soles cleaned with alcohol or sterile water and the... Continue Reading
ABSTRACT Samples of well water were collected from four (4) different wells located in different parts of New Haven in Enugu urban area, for bacteriological analysis. Total bacterial counts, using pour plate method were carried out. Nutrient agar plates were used for general bacterial count, while MacConkey agar plates were used for... Continue Reading
ABSTRACT This work was carried out to investigate the chemical properties of the water extract of zobo (Hibiscus sabdariffa) drink using the modern atomic absorption spectrophotometer. Zobo drink is a popular drink in the Nigeria, especially in the northern part of Nigeria. The result of the analysis indicated that the drink is high in vital... Continue Reading
INTRODUCTION Chlamydiae are small gram-negative obligate intracellular microorganisms that preferentially infect squamocolumnar epithelial cell (www. Google. Com.2010). Chlamydia is the most prevalent sexually transmitted disease resulting in urethritis, with frequencies exceeding those of Neisseria gonorrhoeae. In the United State for... Continue Reading
ABSTRACT Ethanol, Bleach and Phenolics are three kinds of disinfectants which have been widely used in common laboratories. In this study, a compared experiment on these three disinfectants efficiency was conducted against Staphylococcus aureus and Pseudomonas aeruginosa using agar hole diffusion method. Different concentrations of bleach (1%,... Continue Reading
ABSTRACT Three types of sachet water samples and three types of borehole water samples all from Owerri Municipal, Imo State were collected and analyzed for physicochemical parameters. A total of twenty (21) parameters including Odour, Colour, pH, Conductivity, Acidity, Alkalinity, Total Solids, Dissolved Solids, Suspended Solids, Dissolved... Continue Reading
TABLE OF CONTENT CHAPTER ONE 1.0 INTRODUCTION 1.1 AIMS AND OBJECTIVES OF THE RESEARCH CHAPTER TWO 2.0 LITERATURE REVIEW 2.1 SCIENTIFIC CLASSIFICATION OF SENNA TORA 2.2 DESCRIPTION OF SENNA TORA 2.3 SOME COMMON NAMES OF SENNA TORA 2.4 GEOGRAPHICAL DISTRIBUTION OF SENNA TORA 2.5 GROWTH REQUIREMENTS OF SENNA TORA 2.6 ... Continue Reading
ABSTRACT This work evaluates the antioxidant potential of Monodora myristica (African nutmeg). Monodora myristica extract was obtained by solvent extraction using n-hexane and used as treatment on freshly prepared crude palm kernel oil and palm oil. Equal volume of oil samples were subjected to different concentration of extract treatment... Continue Reading
TABLE OF CONTENTS CHAPTER ONE INTRODUCTION 1.1 Herbs 1.2 Objectives of study CHAPTER TWO LITERATURE REVIEW 2.1 History of Piper methysticum (Awa) 2.1.1 Chemistry of Awa 2.1.2 Uses of Piper methysticum 2.1.3 Effect of Awa on the fungal growth 2.1.4 Hepatotoxicity of Piper methysticum 2.1.5 Phytochemicals of Piper methysticum CHAPTER... Continue Reading
TABLE OF CONTENT CONTENT TITLE PAGE CERTIFICATION DEDICATION ACKNOWLEDGEMENT ABSTRACT TABLE OF CONTENTS CHAPTER ONE 1.0 PREAMBLE 1.1 INTRODUCTION 1.2 OBJECTIVE OF STUDY 1.3 SCOPE OF WORK CHAPTER TWO 2.0 LITERATURE REVIEW 2.1 FATS AND OILS 2.2.0 COMPONENTS OF MUCUNA SEEDS 2.2.1 NUTRITIONAL PROPERTIES 2.2.2... Continue Reading
ABSTRACT The extraction of alkaloid from Gaxcina kola (bitter Kola), Kola acuminated (Oji Igbo) and Kola Vera (Oji Hausa) were carried out using 10% enthanoic acid and 10% ethanol to separate the alkaloid from the residue. The alkaloid was extracted using 5% ammonium hydroxide. From the sample results obtained on alkaloid for the sample 9.16%... Continue Reading
TABLE OF CONTENTS CHAPTER ONE 1.0 INTRODUCTION. 1.1 AIMS AND OBJECTIVES CHATER TWO 2.0 DESCRIPTION OF YEAST 2.1 SCIENTICCLASSIFICATION OF YEAST 2.2 HISTORY OF YEAST 2.3 GROWTH 2.4 ECOLOGY 2.5 REPRODUCTUION 2.6 USES OF YEAST 2.6.1 ALCOHOLIC BEVERAGE 2.6.2 DESTILLED ALCOHOL 2.7 GENERAL DESCRIPTION &... Continue Reading
TABLE OF CONTENTS CHAPTER ONE 1.0 INTRODUCTION CHAPTER TWO LITERATURE REVIEW 2.0. SENA TORA 2.1.1 TAXONOMY OF SENNA TORA [NATURE SERVE, 2007] 2.1.2 GEOGRAPHICAL DISTRIBUTION OF SENNA TORA 2.1.3 GROWTH REQUIREMENTS OF SENNA TORA 2.1.4 PROPAGATION OF SENNA TORA 2.1.5 IMPORTANCE USESOF SENNA TORA 2.2.... Continue Reading
ABSTRACT Presence of Escherichia coli from water supplied by water tanker suppliers in Enugu City was determined. Out of eight different water tanker supplies examined which were designated alphabetically as A, B, C, D, E, F, and H. Escherichia coli was isolated from four tanker suppliers. The membrane filtration technique was used and the... Continue Reading
TABLE OF CONTENTS CHAPTER ONE 1.0 INTRODUCTION CHAPTER TWO 2.0 LITERATURE REVIEW CHAPTER THREE 3.0 MATERIALS AND METHODS 3.1 SAMPLE COLLECTION 3.1.1 STERILIZATION OF MATERIALS 3.1.2 PREPARATION OF ERYTHROCYTE FOR HAEMAGGLUTINATION INHIBITION TEST (CHICKEN RED BLOOD CELL) 3.1.3 HAEMAGGLUTINATION ASSAY FOR (STOCK ANTIGEN)... Continue Reading
TABLE OF CONTENTS CHAPTER ONE INTRODUCTION CHAPTER TWO LITERATURE REVIEW DEVELOPMENTAL CYCLE OF CHLAMYDIA MORTALITY/MORBIDITY CHLAMYDIAL URETHRITIS MODE OF TRANSMISSION EPIDEMIOLOGY AND PATHOGENESIS DIAGNOSIS PREVENTION AND CONTROL TREATMENT/THERAPY CHAPTER THREE MATERIALS AND METHODS MATERIALS CHAPTER FOUR RESULTS CHAPTER FIVE... Continue Reading
ABSTRACT Bacteria contamination on Nigerian currency evaluated by exposure method. Pure colony of each isolates were identified using colonial morphological characteristics, as well as biochemical reaction. Nutrient agar, blood agar and sabouraud dextrose agar were exposed for 5 minutes in various Nigerian currency mention in chapter 3. Seven... Continue Reading
TABLE OF CONTENTS CHAPTER ONE 1.0 INTRODUCTION CHAPTER TWO 2.0 LITERATURE REVIEW 2.1 FERMENTED FOODS 2.2 Pentaclethra Macrophylla 2.3 Botany 2.4 ECOLOGY 2.5 Growth and Development 2.6 Method of Production 2.6.1 Microorganism involved in ugba fermentation 2.6.2 Ugba... Continue Reading
TABLE OF CONTENT COVER PAGE DEDICATION ACKNOWLEDGMENT CHAPTER ONE: GENETICS GENE STRUCTURE OF A GENE TRANSCRIPTION TRANSLATION DNA REPLICATION AND INHERITANCE CHAPTER TWO: BENEFITS OF GENETICS GENETIC TECHNOLOGY¬¬ BENEFITS ON INDUSTRIAL BENEFIT ON AGRICULTURE BENEFIT ON MADICINAL GENETIC ENGINEERING CHAPTER THREE: HARMFUL OF GENETICS... Continue Reading
ABSTRACT A total of 400 urine samples were collected between January-August, 2010 among residents of Emene, Ujodo L.G.D.C of Enugu State. The samples were analysed using standard method- macroscopy for the presence of blood and microscopy for the the ova of schistosomes. Results revealed that 5 of them had the infection- 4(1.0%) and 1(0.25%)... Continue Reading
ABSTRACT Locally brewed beer (sorghum beer) is an indigenous alcohol average. It is produced mainly from sorghum grain (Sorghum vulgare ) and the process of brewing involves malting, mashing, souring, fermentation and packaging. Locally brewed beer without hops has a short shelf-life. The aqueous extract of a tropical plant, bitter-leaf was used... Continue Reading
ABSTRACT Potassium bromate is an additive widely employed by bread makers to improve bread quality. On account of its deleterious effect and carcinogenicity in humans, certain levels of potassium bromate are not allowed in bread. Use of potassium bromate in bread is banned in many countries including Nigeria. The present evaluation was carried out... Continue Reading
TABLE OF CONTENTS CHAPTER ONE 1.0 INTRODUCTION CHAPTER TWO 2.0 LITERATURE REVIEW 2.1 PRODUCT DESCRIPTION 2.2 PRODUCTION OF YOGHURT MATERIALS 2.3 VARIETIES IN YOGHURT PRESENTATION 2.4 HEALTH BENEFITS OF YOGHURT 2.5 NUTRITIONAL PROFILE OF YOGHURT 2.6 FACTOR THAT ALTER THE QUALITY OF YOGHURT 2.7 ... Continue Reading
ABSTRACT Isolation and characterisatics of an antibiotics producing bacterium collected from the soil near the arbatior in artesan market Enugu was achieved by characterizing a collection of 74 soil bacteria, including eubacteria and actinomycetes, according to their ability to produce antibacterial antibiotics and their growth responses to 43... Continue Reading
ABSTRACT The purpose of this research was to assess the hygienic properties of commercially available dairy foods especially fresh/raw milk. Milk, when synthesized in the udder of a healthy cow is virtually sterile. As milk passes through the teat cistern and teat channel, it may be contaminated with low levels of bacteria (... Continue Reading
ABSTRACT Microbial evaluation of twenty samples of raw milk from a diary farm (Emene fulani cattle rearers) was carried out using five method: viz direct microcopies count nutrient agar count, Blood agar count, Mac conkey agar count (celiforms only) and Acid fast bacilli staring was done to assay for the presence of the Tubercle bacillus. The... Continue Reading
ABSTRACT The prevalence of Trichomonas Vaginalis among adult in “Osumenyi†in Nnewi south local government Area of Anambra state was carried out. This was done using a stotal of one hundred adults from there different clinics viz ;- Health center, family planning and Antenatal Clinic in Osumenyi; in Nnewi South L.G.A of Anambra... Continue Reading
ABSTRACT This work is aimed at determining the incidence of bacterial injections such as Salmonella and Escherichia coli which cause health hazards to the poultry in their feeds. Sample feed were obtained from commercial mash operation/ pellet mill operation at New heaven and top feed at Ogbete main market Enugu. The samples were screened... Continue Reading
ABSTRACT In thus work the incidence of candidacies was studied using selected patients of university of Nigeria teaching hospital Enugu analyzed for the presence of candidacies yeast infection. The specimens were cultured on seaboards agar and blood agar and subculture isolated were C aibicans C Tropical C Knusei C Pseudoizo P cals C paraknusei... Continue Reading
ABSTRACT In the work, samples of Garri obtained from Ogbete market were evaluated for microbial contamination. Four sample of Garri from different sources of supple were collected from Ogbete main market Enugu Okwo, Nkalagu, Emene, and Abakaliki respectively. The sample were extracted using equal volume of sterile distilled water and serially... Continue Reading
ABSTRACT The Anatomy of this project write-up encompasses the ordinary look of enzymes as organic molecules, rather it sheds more light into the comprehensive view and determination of the catalytic and specific activity enzymes particularly- DIASTASE, MALTASE and ZYMASE can exploit in industrial application, also critically important is the... Continue Reading
ABSTRACT Onion is associated with micro-organisms which are capable of causing spoilage. Onions with rot were examined microscopically. A solution of it was made and the serial dilution of 1:10-1 to 1:1-5 were made from 10ml of the onions sample. From the dilution, 1ml of each diluent was plated on nutrient and saboor and dextrose agree to... Continue Reading
ABSTRACT This research work was on maize (Zea may) abject in sorghum vulgare) beer brewing the sorghum was malted and milled. Malting was achieved by sleeping the grains in water at 280c for 48 germination of the grain was allowed for 72 hrs and kilmed in air oven at 550c for 48 hrs. maize as an adjuct was in corporate into the malted milted... Continue Reading
ABSTRACT The isolation and identification of bacteria associated with 150ml sepsis studies were carried out to 250 patient at National Orthopedic Hospital Enugu. (250) Two hundred and fifty patients with 150ml sepstis was grouped in to in patients and out patients. The sample collected are wound pus and wound exudates which were collected... Continue Reading
ABSTRACT The microbial changes which took place during the steeping and storage of pan (ogi) was studied. The steeped water had an initial PH of 6.8 which latter reduced to 4.9 at the end of steeping. The bacterial number for the steeped water ranged from 4.7x104 to 3.2 x 107 cfu/ml while the fungal number ranged from 1.5 x103 to 5.7 x 106... Continue Reading
ABSTRACT This project work is carried at the isolation and characterization of pathogenic bacteria from hawked suya meat, were bought in full from the sellers and was collected in raps of aluminum foil. It was then taken to the laboratory for analysis the samples were homogenized and serial dilution of the sample was made out using 5 ml sterile... Continue Reading
ABSTRACT A total of fifty faecal samples were collected form pupils in airport primary school Emene Enugu were used for the study. The duration of the investigation was between February August 2005. These samples were cultured and their causative organisms determined using macconkey agar, deocycholate citrate agar and an alkaline peptone water... Continue Reading
ABSTRACT This project work which is all about the fungal infestation of bakery product BREAD, explain the kind or type of fungal organisms that are responsible for the spoilage on contamination of bread. The infestation of fungal organism on bread depends on the kind and number of the agent present and upon the environment about them. this... Continue Reading
ABSTRACT The incidence of the infestation of plasmodium species (P.Falciparum, P. Vivax, P.Ovale, and P. alaria) which causes human malaria in Enugu metropolis was conducted at Parklane Hospital using their patients. Thick and thin, smear of blood were made and examined after proper staining. The staining method which proved effective were... Continue Reading
ABSTRACT A study was carried to ascertain the potency of some techniques used in diagnosis of Human Immunodeficiency virus (HIV). Two hundred and five samples (203) were collected from the patients from the university of Nigerian teaching hospital and was analysed using Elisa kit, which has the ability to detect antibodies and antigens in the... Continue Reading
ABSTRACT Samples of water from the different hostels in campus II and other source of water production namely Ajali-owa water, from bore hole 9th mile and spring water from GRA water board were water collecterd and analysed using total plate count and multiple tube fermentaton techniques coliform. The results shows no coliforms in production... Continue Reading
ABSTRACT In comparing the antimicrobial strength of three most common antibiotics, which includes: streptomycin chloranphenicol and gentarccin. Samples of urine and high vaginal swab (H.V.S) were collected from park lane Enugu. The organisms isolated were pure culture of staphylococcus aureus and Escherichia coli Sensitivity test was carried... Continue Reading
ABSTRACT The study on anaemia in pregnancy is aimed at determining packed cell volume (PCV), Haemoglobin (Hb) level and Erythrocyte sedimentation rate (ESR) of pregnant women, as well as the selferity, significance of maternal, age, educational level, occupation and gestational age to the occurrence of anaemia in pregnancy managed, diagnosed and... Continue Reading
ABSTRACT The survey of urinary sehistomiass in Ishele in Amagunze, Nkanu East local Government was carried out using two methods of diagnosis i.e combination of questionnaire on clinical signs and symptoms, and laboratory microscopy using primary school children in the area as test samples. A total of fifty samples were randomly collected reveals... Continue Reading
This project work is aimed at investigating whether there is a relationship between weight and blood pressure of patients with high blood pressure. Background of the Study The number of people with high blood pressure is in the increase and research has shown that this condition is heightened by overweight of the patient. That is to say, the more... Continue Reading
Yoghurt is a cultural diary product produced by lactic fermentation of milk (Hui, 1992). Yoghurt is produced by the controlled fermentation of milk by two species of bacteria; Lactobacillus bulgaricus and Strptococcus thermophilus. These two species of bacteriae have now been established as the yoghurt starters. Any sort of milk may be used to... Continue Reading
ABSTRACT Newcastle disease (ND) is one of the most important avian viral diseases because of its high economic impact on the poultry industry. Newcastle disease is now claimed to be responsible for 70-80% of annual death in village chickens. Losses in hundreds of poultry birds of various ages and sexes in Anyigba Kogi State of Nigeria is... Continue Reading
ABSTRACT Ugba (Ukpaka) is the Ibo name of the fermented African Oilbean seeds (Pentaclethra macrophylla, Benth). It is a popular traditional food condiment in Nigeria especially among Ibo ethnic group generally. It is produced by natural (local) fermentation in homes as a small family business. It is an important and cheap source of protein for... Continue Reading
ABSTRACT Juice was extracted from banana (Musa sapientum) pulp with the addition of lemon juice and was inoculated with Baker’s yeast (Saccharomyces cerevisiae) and held at 30 for seven days. The result of the yeast count increases at 48hr, and at 96hr the yeast count decreased gradually. It ranges from 4.9x107 cfu/ml at 0hr, 5.1x107 at... Continue Reading
ABSTRACT Twenty (20) samples of suya meat in Enugu were collected randomly and analyzed microbiologically and the isolates were identified as Staphylococcus aureu (35%), Escherichia coli (15%), Streptococcus species (15%), Pseudomonas (35%). The most frequently isolated organisms were Staphylococcus and Pseudomonas. The total viable bacterial... Continue Reading
ABSTRACT The bacterial contaminants associated with commercial poultry feeds from three companies in Nigeria (vital, Guinea and Top) were studied using streak plate techniques. The culture media used were Nutrient agar and Mac Cokey agar. The aim/ objective of the study is: To ascertain the microbial safety of commercial poultry feeds produced... Continue Reading
ABSTRACT The effect of pesticides, chchlorodipheny trichloroethane (DDY) and Benzene hexaxhoride (BHc) on the microflora of the three types of soil (loamy, clay and sandy soil) was conducted. The serial dietitian technique was employed up to 10-3 using nutrient agar, potato dextrose agar and sabourand agar plates. The isolation of both bacterial... Continue Reading
ABSTRACT The antibacterial activity of three type of medicate soap on staphylococcus aureus isolated from wound infections was conducted. Fifty individual with would infection within the age range of 9-73 years were sampled. Swabsticks were used to collect specimens from wound infection. each swabstick was streaked separately over plates of... Continue Reading
ABSTRACT Rotavirus is a common cause of diarrhea in children. This study was carried out to determine the prevalence of rotavirus antigen in children below five years presenting with diarrhea in Gombe State. Thirty (30) stool samples were analysed using ELISA KIT. Out of the total number of samples analyzed, a prevalence of 6.67% was recorded... Continue Reading
ABSTRACT Milk, Yoghurt and Cheese are highly valuable food which are readily digested and have high concentration of nutrients which have proved to be a heaven of microbes, studies were conducted on the isolation and identification of microbes [Fungi and Bacteria] on peak canned milk, yoghurt, locally prepared cheese and cheese water. It was... Continue Reading
ABSTRACT Pleurotus species are popularly known as oyster mushroom which are regarded as one of the edible mushroom of commercial important throughout the whole world. Utilization of this depends on the ability to secrete cellulolytic and hemilocellulolytic enzymes which can make use of wide spectrum of Agro industrial wastes for the growth and... Continue Reading
Four medicated soaps (Ghana soap, Tetmosol, Beneks’, and Crusader) were investigated for their antibacterial activities against Staphylococcus aureus and Staphylococcus epidermis. A total number of thirty (30) students of Western Delta University, Oghara, Delta state, 15 males and 15 females were skin swabbed with sterile swab sticks.... Continue Reading
ABSTRACT The study determines the antifungal effects of aqueous, ethanolic and methanolic extracts of Garcinia kola on some selected fungal isolates and their phytochemical constituents. The antifungal sensitivity and Minimum Inhibitory Concentration (MIC) were determined by agar well diffusion and agar dilution methods, respectively using... Continue Reading
ABSTRACT Infection with Chikungunya virus (CHIKV) is highly infectious disease that causes some dermatological manifestations and clinical symptoms. CHIKV is a member of the family Togaviridae. Structurally, it has an encapsidated genome composed of single-stranded RNA. This study is carried out to determine the seroprevalence and prevalence of... Continue Reading
ABSTRACT The study involved the isolation of (2) isolates titled RMN1, RMN2, belonging to the Genus Serratia from ( 2) soil samples collected from the following two strategic locations in university of Abuja, permanent site: Termite hill besides Girls hostel dump ground and from Faculty of Agricultural Science farm land. The isolates were... Continue Reading
ABSTRACT This research work involve the isolation of two isolates titled RMN1 and RMN2, belonging to the Genus Serratia from two soil samples collected from the following two strategic locations in university of Abuja, permanent site: Termite hill besides Girls hostel dump ground and from Faculty of Agricultural Science farm land. The... Continue Reading
INTRODUCTION Dairy products are various products derived from cow’s milk or that of other female mammals such as goat, sheep, yaks, horses, camel. Dairy products include yoghurt, nono (fermented cow’s milk, madara (unfermented cow’s milk, cheese, whey, condensed and evaporated milk.) (cultureforhealth, 2015). Yoghurt, nono, Madara has become... Continue Reading
Abstract Abstract Background: Occult hepatitis B infection (OBI) is defined as serologically undetectable hepatitis B surface antigen (HBsAg-ve), despite the presence of circulating HBV DNA (Allain, 2005). It has been documented that one of the frequent complications of HIV infection is hepatitis B co-infection and due to the common methods of... Continue Reading
ABSTRACT The comparative evaluation of the bacterial and fungal diseases associated with Saccharum officinarum (Sugarcane) in ethnobotanical garden of Wesley University, Ondo was carried out through different microbial processes. The objectives of this work were to comparatively evaluate bacterial and fungal diseases associated with sugarcane in... Continue Reading
ABSTRACT Honey has since been found to possess antibacterial property and is therefore employed for wound and gastro-intestinal disease therapy. This study evaluates the antibacterial spectrum and efficacy of honey and compared with tetracycline. Different concentrations (50, 75 and 100.0 %) of honey were studied in-vitro using Staphylococcus... Continue Reading
ABSTRACT The castor plant is a robust perennial shrub of Euphorbiacaea family. The seeds are documented to have an antibacterial profile against some bacterial organisms such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus vulgaris, and Streptococcus pyogenes. ... Continue Reading
ABSTRACT A total of 100 subjects were screened for S. aureus strains, thirty (30) S. aureus isolates were obatained. The carriage of β-lactamase among the 30 isolates was determined by using starch iodine agar plate method. It was found that 19(63.3%) of the S. aureus strains were β-lactamase producing. Plasmid curing of the β-lactamase... Continue Reading
ABSTRACT The microbiological and physicochemical characteristics of soil receiving palm oil processing waste (POPW) in Abraka were investigated. The effect of palm oil processing waste was tested on loamy, sandy and clay soils. The pour plate technique was used for the isolation of the organisms. The total mean bacterial count ranged from... Continue Reading
ABSTRACT Zobo and Kunu drinks are non-alcoholic beverage that is widely produced and consumed in Nigeria.The beverage may possess health risk when contaminated due to method of preparation. Zobo and Kunu drinks were purchased from different sellers in Olabisi Onabanjo University, Ago-Iwoye Ogun State. Physical and chemical analysis of the drinks... Continue Reading
ABSTRACT The study was conducted to assess the antibacterial activity of Phyllanthus amarus (Schum and Thonn) extract against Salmonella typhi causative agent of typhoid fever at the laboratories of the Departments of Chemistry and Theoretical and Applied Biology of the College of Science, Kwame Nkrumah University of Science and Technology,... Continue Reading
ABSTRACT was studied. The leave was observed to be highly rich in polyphenols, flavonoid, reducing compounds and alkaloids. Glycosides, saponins and tanins were present in small amount, there was absence of Phlobotanins,... Continue Reading
ABSTRACT Phytochemical and Antimicrobial activity of the leaves of Tetrapluera tetraptera using aqueous and ethanolic extract was studied. The leave was observed to be highly rich in polyphenols, flavonoid, reducing compounds and alkaloids. Glycosides, saponins and tanins were present in small amount, there was absence of Phlobotanins,... Continue Reading
To determine the presence of microorganisms present in the indoor air coming from out air conditioners. CHAPTER ONE 1.0 INTRODUCTION 1.1STATEMENT OF PROBLEM 1.2SCOPE OF WORK 1.3JUSTIFICATION 1.4AIM AND OBJECTIVE CHAPTER TWO 2.0LITERATURE REVIEW 2.1COMMON FUNGI ISOLATED FROM INDOOR AIR 2.1.1ASPERGILLUS SPP... Continue Reading
ABSTRACT The presences of pathogenic microorganism in ice cream such as Salmonella Species, Staphylococcus aureus, Escherichia coli have been well documented, but examination of pathogenic bacteria like Bacillus cereus Yersinia enterocolitica, Brucella species and E.coli O157:H7 have been rarely studied. These studies indicated... Continue Reading
Medicinal plants have provided a source of inspiration for novel drugs compounds since plant derived medicines have made large contributions to human health. The traditional healers make use of water primarily as solvent but extracts of these plants were certainly much better and powerful. This may be due to the better solubility of the active... Continue Reading
ABSTRACT Endophytic microorganisms have proven to be potential sources of antimicrobials against recalcitrant pathogens. Extracts of an endophytic fungus, Emericella nidulans exhibited antibacterial activity against selected pathogens. Emericella nidulans was cultivated in broth and fermented at 28 ± 2°C for 7days and 21 days... Continue Reading
ABSTRACT This study was carried to with the aim of determining the antibacterial effect of Calotropis procera against some bacteria isolated spoilt nunu. Bacteria isolated from spoilt nunu were identified based on their Gram characteristics, morphology and biochemical characteristics. Calotropis procera was extracted using ethanol, methanol and... Continue Reading
ABSTRACT The human hands harbour microorganisms both as part of a person’s normal microbial flora as well as transient microbes acquired from the environment. This study was carried out to isolate and identify Bacillus spp that are associated with the hands of apparently healthy female undergraduate students in Obafemi Awolowo University. The... Continue Reading
Nine samples of freshly prepared kunu was taken from local vendors and hawkers at Tanke area, Ilorin, Kara State and analyzed for microbial quality. The pH of the samples ranged from 2.66 to 4.06. The total bacterial count ranged from 1.5×104 to 7.4×104 cfu/ml; the total coliform count ranged from 1.0x102 to 8.0x103cfu/ml; the faecal coliform... Continue Reading
ABSTRACT were carried out using soil samples collected from different locations at Osun State University, Osogbo, main campus. The soil samples were collected from twelve (12) diffeferent locations within the study site. The samples were cultured aseptically by inoculating each samples on... Continue Reading
Aquaculture has been a growing activity for the last 20 years worldwide and this impressive development has been attended by some practices potentially damaging to animal health. The bacterial infections are considered the major cause of mortality in aquaculture. Among the common fish pathogens, A. hydrophila and Y. ruckeri as gram-negative and S.... Continue Reading
ABSTRACT Mushroom has been an important food to man for millennia. Several species of Pleurotus has been cultivated successfully on different substrates including Palm bunch, Saw dust and Corn cob. Pleurotus pulmonarius has also... Continue Reading
ABSTRACT Hepatitis C virus (HCV) is a meticulous factor of liver disease and one of the most important health issues worldwide. Hepatitis is the inflammation of the liver produced by autoimmune disease, alcohol or drug abuse, genetic disorder or microbial infection. To detect the presence; prevalence; incidence; and the relationship between the... Continue Reading
ABSTRACT In African folk medicine, water infusion of Mangifera indica stem bark are highly valued in thelocal treatment of malaria fever, diarrohea, diabetes mellitus and liver diseases. Various part ofthis plant can also be used in treatment of toothache, dysentery, anemia, hypertension andinsomnia in... Continue Reading
ABSTRACT The present experiment aims at investigating the growth behaviour of different yeast strains inselected carbon, nitrogen and stress environment to obtain strains with prospects for industrialapplication. Specifically, the study is set to: isolate yeast from decaying mango fruit ... Continue Reading
Microbiological tests revealed that Hibiscus sabdariffa plant extract has antibacterial properties thus verifying folklore medicine in the treatment of abscesses, bilious conditions, cough, dysuria and scurvy (Morton, 1987; Perry, 1980 Ross, 2003). The extraction type of aqueous ... Continue Reading
ABSTRACT This research work was carried out to examine the physicochemical and phytochemical constituents of honey and Shea butter samples respectively and their antibacterial effect on Staphylococcus aureus and Klebsiella pneumoniae . The physicochemical screening of honey showed that the honey samples used have low water activity, low moisture... Continue Reading
ABSTRACT In the for anti microbial effect the back peel of Persea americana (AVACADO) was investigated for activities . the study was to evaluate the antimicrobial efficacy of the crude ethanolic and aqueous extract of the peel of Persea americana against selected clinical isolates. The agar well diffusion method was used in study also... Continue Reading
The study was carried out to investigate the effect of aqueous and petroleum ether extract of momordica charantia on blood glucose level of albino rats.The extraction was done using petroleum ether and water .Thirty two albino rats were grouped into eight (A ,B,C,D,E,F,G,H). Comprising of four in each group .Diabetes was induced with a single Dose... Continue Reading
ABSTRACT Chlorella vulgaris is an edible microalgae and a highly notorious potential feed resource for many agriculturally important animal species. Chlorella vulgaris intake has also been linked to improvements in animal health and welfare. Its influence over animal development stems from its nutritive and protein-rich composition, thus... Continue Reading
ABSTRACT The safety, efficacy and quality of herbal mixtures have been an important concern for health authorities and health professional, especially now there is increase in the use of herbal mixtures. This study was aimed at isolation and identification of microorganisms from some liquid herbal mixtures sold in Enugu metropolis, South East of... Continue Reading
ABSTRACT Petroleum – polluted sites are disturbed ecosystem with scanty plants. Study was carried out in such sites to assess the area of its rhizobacterial status, particularly comparing that of the rhizospheres and non-rhizospheres in the site. The samples were suspended and serially diluted in physiological saline and inoculated on nutrient... Continue Reading
ABSTRACT Diarrhoeal diseases in Kenya are among the five main causes of mortality in children younger than five years. Bacterial diarrhoea has been reported to account for up to 30 % of all cases of infantile diarrhoea. Among children of age five years and below, diarrhoeagenic E. coli (DEC) such as enterotoxigenic E. coli (ETEC), enteropathogenic... Continue Reading
ABSTRACT Global crop and energy production are fast dwindling inversely to population growth. Common bean (Phaseolus vulgaris L.) yield has reduced worldwide due to soil infertility which can be reversed through application of chemical fertilizers. The chemical fertilizers used to ameliorate nitrogen, phoshorus and potassium are expensive and... Continue Reading
ABSTRACT This research was conducted in Microbiology Laboratory of Godfrey Okoye University to determine the antimicrobial efficacy of aqueous, methanol, ethanol of Pisdium guajava and Citrus x sinensis on the pathogenic Streptococcus mutans and Candida albicans . The Dimethyl sulfoxide was used for dissolving the plant extracts. Pisdium... Continue Reading
ABSTRACT The evaluation of power density of ray emissions from GSM masts in selected locations within Ilorin metropolis were carried out. The measurements taken in each spot were done using Acoustimeter. The location that shows the highest peak measurement (5.12 ± 1.07) location H, which exceeded the international approved exposure limits which... Continue Reading
ABSTRACT Polyhydroxybutyrate (PHB) are biodegradable materials, which are accumulated to store carbon and energy in various microorganisms. Soil samples used for the study were collected from groundnut farm garden. The isolates were screened for PHB production using sudan III... Continue Reading
Abstract Indigenous bacteria in the soil environment can produce a wide range of solvents and metabolites more efficiently. This study was carried out to screen Clostridium species isolated from the soil environment within Jos metropolis for solvent production. A total of 30 samples were collected from farmlands, nursery gardens, floral gardens... Continue Reading
ABSTRACT Background: The pathogenesis of cerebral malaria (CM) is still recognized as multifaceted, despite the plausible explanation provided by the high degree of sequestration of parasitized erythrocytes in major organs of the body. Postmortem studies have demonstrated the presence of hemorrhage, deposition of fibrin and clots in blood vessels... Continue Reading
ABSTRACT BACKGROUND: Extended Spectrum Beta-Lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae are pathogens of significant public health interest to which new antibiotics therapies are urgently needed. AIM: This study was designed to determine the prevalence of ESBLs in clinical isolates of E. coli and K. pneumoniae from... Continue Reading
ABSTRACT This research was conducted in Microbiology Laboratory of Godfrey Okoye University to determine the antimicrobial efficacy of aqueous, methanol, ethanol of Pisdium guajava and Citrus x sinensis on the pathogenic Streptococcus mutans and Candida albicans . The Dimethyl sulfoxide was used for dissolving the plant extracts. Pisdium... Continue Reading
ABSTRACT Extended-spectrum beta-lactamases (ESBL) are enzymes that confer resistance to most beta-lactam antibiotics, including penicillin, cephalosporin, and the aztreonams. The aim of this present study is to phenotypically identify and establish the presence of ESBL-producing organism among students in the university community. Within the... Continue Reading
ABSTRACT Normal vaginal flora contains a wide range of microorganisms. Bacterial vaginosis BV is the main reason for vaginal discharge. Many gram-positive and gram-negative rods i.e. E.coli, Klebsiella , Proteus, Acinetobacter and Pseudomonas spp. are major contributors to bacterial vaginosis. Aim: The present study was conducted to elucidate the... Continue Reading
Introduction: Prevalence of resistant microbial strains is increasing with time; due to the extensive and indiscriminate use of the broad spectrum antimicrobial drugs that render the current used antimicrobial agents insufficient to control some microbial infections. Objective: The present study was aimed to investigate the antimicrobial and... Continue Reading
ABSTRACT Hepatitis B Virus (HBV) occurs worldwide with more than 2 billion people being infected at some time in their lives. Transmission of this virus from carrier mothers to babies can occur during perinatal period. This study was conducted to determine prevalence of HBV infection among pregnant women in Rea Sea State. The study was carried out... Continue Reading
Abstract This was analytical study conducted during the period from January to April 2017, to determine the antimicrobial activity of Azadirachta indica leaves methanolic extract against different bacteria isolated from urinary tract infection. A total of 130 urine samples were collected from patients with Urinary tract infection. These specimens... Continue Reading
Pyrexia of unknown origin (PUO) is fever that occurs for no apparent reason with failure toestablish diagnosis within one week of investigation. The fever involves temperatures ofgreater than 38.3oC which occurs sporadically for more than three weeks. Fever resolvesusually after about 3 days but can take 2 weeks. Dengue is a tropical mosquito... Continue Reading
ABSTRACT In a research conducted to ascertain the antimicrobial activity of watermelon (Citrullus lanatus) bark on organisms such as Escherichia coli, Slamonella typhi and Staphylococcus aureus using the ethanol and auqous extracts of the watermelon bark applying agar-well and pour-plate sensitivity technique. It was observed that the ethanoic... Continue Reading
ABSTRACT The effect of spent engine oil on soil properties and growth of Spinach (Spinacia olereacea) was investigated. Five treatments (0, 20, 40, 60, 80)ml of the spent oil were applied to soil in perforated poly bags with Spinach strands at four weeks after stabilization. Soil analysis showed that spent engine oil had no effect on both the pH... Continue Reading
ABSTRACT The study assessed the prevalence, intensity and environmental risk factors affecting the transmission of soil transmitted helminthiasis in eleven randomly selected communities in Nsukka and Uzouwani Local Government Areas of Enugu State, Nigeria. It also determined the effect of peoples’ knowledge of mode of transmission of the... Continue Reading
ABSTRACT The prevalence of streptococcus pneumonia in pneumonia patients using university of Nigeria Teaching Hospital (UNTH) Enugu as a case study was carried out based on the fact of the pneumonia is one of the commonest afflictions of the aged people and children worldwide. A total of 50 samples were collected. 12 (24%) of the sample were from... Continue Reading
A total number of 60 samples of water leaf were collected from different areas in Enugu Metropolis, these samples were inoculated and examined to detect the presence of sorbitol negative Escherichia coli 0157:H7. About 35(17.5%) samples out of the 50 samples inoculated were found to be sorbitol negative E-coli. From the result E-coli 0157:H7 is... Continue Reading
Spondias mombin (Linn.) is a widely cultivated edible plant used in folkloric medicine for the treatment of severe infection and health disorders. This research work was carried out to isolation, purification and chemical characterization the bioactive constituents of the ethyl acetate stem bark fraction of Spondias mombin (Linn.), a medicinally... Continue Reading
The purpose of this research work is to investigate the elemental composition and antioxidant potentials of Spondias mombin (leaf, root and stem bark) at concentration of 10, 20 and 5mg/ml. using different conventional laboratory methods, also to determine the antioxidant potentials of Spondias mombin. The element present are Sodium (Na), Calcium... Continue Reading
Abstract The main purpose of this work is to investigate and compare the antimicrobial properties of Chrysophyllum albidum seed and stem bark essential oils, against some selected pathogenic isolates, to compare the phytochemical composition of the oil of Chrysophyllum albidum seeds and stem bark, to analyze the chemical compounds responsible for... Continue Reading
Abstract The purpose of this present study is to determine the antibacterial potency of essential oil of Polyalthialongifolia, to determine the presence of phytochemicals and structural determination of bioactive component from the leaf portion using GCMS In this present study, the antibacterial ... Continue Reading
Abstract This study aims to determine the phytochemical characteristics, structural determination of bioactive component from the leaf extract using GCMs and experimentally investigation of the antibacterial activity of essential oil from Persea americana and Citrus sinensis leaf against seven bacteria (Gram-positive and Gram-negative) which are... Continue Reading
Morinda lucida is a tropical West African tree of medium-size, having brown colored bark, white flowers and yellowish ellipsoid seeds. It is widely used in traditional medicine systems of different countries. The preliminary phytochemical screening of different extracts prepared from bark, leaves and seeds of M. lucida revealed the presence of... Continue Reading
The plant Morinda lucida falls under the family Rubiaceae known to have wide usage in traditional medicine. Morinda lucida is a tropical West African tree of medium-size about 18–25 m tall, the bark is grey to brown in colour, flowers are white in colour, the fruit is a drupe, seed is ellipsoid, yellowish and soft. The purpose of this work is... Continue Reading
Daniella oliveri (Caesalpiniacea) is a plant found in the Amazon region and other parts of South America and Africa. The tree may reach a height of 100 feet and trunk diameter of 4 feet. Daniella oliveri (Rolfe) Fabiaceae is commonly known as Ilorin balsam (eepo-iya) or Copaihu africana . It is an indigenous African tree, found extensively in... Continue Reading
Erythrina senegalensis DC. (Coral tree) is commonly grown in West Africa as an ornamental plant and one of the oldest known African medicinal plants. The purpose of this research is to determine the antimicrobial potency, phytochemical activities, nutrients and elemental composition of the leaf and bark of Erythrina senegalensis. The organisms... Continue Reading
Morinda lucida is a tropical West African evergreen shrub or small to medium sized rainforest tree, having brown colored bark, white flowers and yellowish ellipsoid seeds. It is widely used in traditional medicine systems of different countries. This study was designed to carry out the preliminary phytochemical screening and evaluation antioxidant... Continue Reading
Bacteria are group of microorganisms all of which lack a distinct nuclear membrane (and hence are considered more primitive than animal and plant cells) and of most which have a cell wall of unique composition. Most bacteria are unicellular; the cells maybe spherical (coccu), rod-shaped (bacillus), spiral (spirillum), comma-shaped (vibrio) or... Continue Reading
Abstract Background Newcastle disease is a highly contagious disease of birds caused by Paramyxovirus. Birds mostly affected by Paramyxovirus virus includes; chickens, turkeys, geese, ducks and guinea fowls. Newcastle disease however, remains a worldwide problem. There are three major strains of Newcastle disease virus and they are velogenic,... Continue Reading
A RESEARCH PROJECT ABSTRACT The study on the isolation and antibiogram of bacterial isolated form sealed and unsealed drugs revealed total Bacteria count ranging form 1.2 X 107 to 11.5 X108cfu/g for unsealed and 1.5 X107 to 9.2 x 107cfu/g for sealed. The following bacterial... Continue Reading
A RESEARCH PROJECT ABSTRACT Antibiotic sensitivity testing is the susceptibility of bacteria to antibiotics, it is carried out to determine which antibiotic will be most successful in treating a bacterial infections. The total mean colony count of the bacterial... Continue Reading
ABSTRACT Culturally, consumption of local fermented skimmed milk (nono/tsala) is common in Northern Nigeria and many other parts of Africa and it is sometimes associated with microbial diseases due to poor hygienic practice. Hence, this study was conducted with the objectives of assessing the microbial quality and some physicochemical parameters,... Continue Reading
ABSTRACT Microorganisms are important components in the structure and function of organic matter in soil, hence this work was done to identify bacterial communities present in flooded soil. Soil samples were collected from eight different areas within Benin metropolis, Edo State, Nigeria. The samples were cultured on using pour plate... Continue Reading
INTRODUCTION Hepatitis B is a viral disease process caused by the Hepatitis B virus (HBV). Hepatitis B virus is a major cause of acute and chronic hepatitis worldwide and is more prevalence in developing countries. More than 2 billion people are infected with HBV worldwide while some 280million are chronic carriers, harbouring the... Continue Reading
ABSTRACT Antibiotic resistance has become a wide spread problem. The efficacy of two solvent extracts of Cymbopogon citratus against some test bacteria and fungi was evaluated. The test bacteria was; Staphylococcus aureus, and the test fungi was; Candida albicans. The phytochemical properties and the antimicrobial potency of Cymbopogon citratus... Continue Reading
Oryza sativa husks and peels of Musa paradisiaca were pretreated by hydrothermal and acid fermentation using cassava mill effluent, and co-digested in a laboratory scale reactor maintained at 45 ± 2 ⁰C for 42 days. Five digesters were used for the experiment and each digester contained equal proportion of Oryza sativa husks and Musa paradisiaca... Continue Reading
DERMATOPHYTE are unique of fungi that effect the Keratinous tissues of lower animals and human Dermatophytoses is the medical term for fungal infection of the superficial layer of the skin, nail, hair and scalp. The prevalence study Dermatophytoses in Wuntin Dada Primary School Bauchi is to determine the prevalence of the diseases, the different... Continue Reading
ABSTRACT This project is basically concerned with the extraction of DNA in tomatoes and garden egg fruit. An experiment on how to extract DNA on tomatoes and garden egg, isolation on it to see what DNA actually look like as well as importance of DNA extraction are consid3ered in this project. TABLE OF CONTENT Title page... Continue Reading
. ABSTRACT Lassa fever is an acute viral illness characterized by multi-organ failure and hemorrhagic manifestations. Lassa fever is most frequently diagnosed in Nigeria, Sierra Leone, Liberia, and Guinea, although sporadic cases have recorded in other West Africa countries,... Continue Reading
ABSTRACT The seed Persea americana was extracted with four different solvents via chloroform,petroleum ether,ethylacetate and methanol and subjected to preliminary phytochemical screening which revealed alkaloids, steroids, saponins, tannins, flavonoids, glycosides, and phenols. Persea... Continue Reading
(CASE STUDY OF AKWUKE CATTLE RANCH IN ENUGU) ABSTRACT The isolation and identification of bacterial organisms in raw milk was carried out. The pour plate technique was employed for isolation using MacConkey agar, blood agar and calf serum agar. Gram-staining and biochemical tests... Continue Reading
ABSTRACT The bacteriological examination of drinking water sourced from Ugbawka Town, Nkanu East Local Government Area of Enugu State was carried out to determine the bacterial load in the water samples and organisms that contaminates the water.... Continue Reading
ABSTRACT Antimicrobial activities of grape fruit seed extract ws investigated. The organisms used includes Staphylococcus aureus and Escherichia coli which belong to gram positive and gram negative organisms.... Continue Reading
INTRODUCTION Hepatitis B is a viral disease process caused by the Hepatitis B virus (HBV). Hepatitis B virus is a major cause of acute and chronic hepatitis worldwide and is more prevalence in developing countries. More than 2 billion people are infected with HBV worldwide while some 280million are chronic carriers, harbouring the... Continue Reading
ABSTRACT The results obtained from this research work shows that land snails sold in Ekpoma market contains some pathogenic microorganisms. A total of fifty samples were bought from 10 different local sellers (five snail samples each) in Ekpoma old market and the new market. The total viable count of bacteria ranged from 2.2 x 108 cfu/g - 8.2 x... Continue Reading
CHAPTER ONE 1.1 INTRODUCTION Plants have provided a source of inspiration for novel drug compounds, as plant derived medicines have made large contributions to human health and wellbeing. Plant extracts have been used for a wide variety of purposes for many thousands of years (Jones, 1996). The antimicrobial activity of plant oils and extracts has... Continue Reading
Medicinal plants have provided a source of inspiration for novel drugs compounds since plant derived medicines have made large contributions to human health. The traditional healers make use of water primarily as solvent but extracts of these plants were certainly much better and powerful. This may be due to the better solubility of the active... Continue Reading
ANTIMICROBIAL ACTIVITIES OF SELECTED PLANTS (BITTERLEAF, UTAZI, AND BITTERKOLA LEAF)EXTRACT’S AGAINST FISH PATHOGENIC BACTERIA TABLE OF CONTENT CHAPTER ONE 1.1 introduction 1.2 objectives the study references CHAPTER TWO 2.1 ethnobotanical 2.1.1 medicinal plants 2.2 bitterleaf 2.2.1 antibacterial properties of bitterleaf 2.3 utazi 2.3.1... Continue Reading
ANTIMICROBIAL ACTIVITIES OF SELECTED PLANTS (BITTERLEAF, UTAZI, AND BITTERKOLA LEAF)EXTRACT’S AGAINST FISH PATHOGENIC BACTERIA TABLE OF CONTENT CHAPTER ONE 1.1 introduction 1.2 objectives the study references CHAPTER TWO 2.1 ethnobotanical 2.1.1 medicinal plants 2.2 bitterleaf 2.2.1 antibacterial properties of bitterleaf 2.3 utazi 2.3.1... Continue Reading
ABSTRACT Microbial analysis was carried out on six samples of toasted bread bought from three different kiosks in Unilorin walkway. The microbial population of each sample was determined and related to a variety of physiochemical parameters that could have contributed to their population. These parameters included the pH which varied from 5.37 –... Continue Reading
ABSTRACT Microbial analysis was carried out on six samples of toasted bread bought from three different kiosks in Unilorin walkway. The microbial population of each sample was determined and related to a variety of physiochemical parameters that could have contributed to their population. These parameters included the pH which varied from 5.37 –... Continue Reading
Four medicated soaps (Ghana soap, Tetmosol, Beneks’, and Crusader) were investigated for their antibacterial activities against Staphylococcus aureus and Staphylococcus epidermis. A total number of thirty (30) students of Western Delta University, Oghara, Delta state, 15 males and 15 females were skin swabbed with sterile swab sticks.... Continue Reading
Spondias mombin (Linn.) is a widely cultivated edible plant used in folkloric medicine for the treatment of severe infection and health disorders. This research work was carried out to isolation, purification and chemical characterization the bioactive constituents of the ethyl acetate stem bark fraction of Spondias mombin (Linn.), a medicinally... Continue Reading
The purpose of this research work is to investigate the elemental composition and antioxidant potentials of Spondias mombin (leaf, root and stem bark) at concentration of 10, 20 and 5mg/ml. using different conventional laboratory methods, also to determine the antioxidant potentials of Spondias mombin. The element present are Sodium (Na), Calcium... Continue Reading
ABSTRACT Four medicated soaps (Ghana soap, Tetmosol, Beneks’, and Crusader) were investigated for their antibacterial activities against Staphylococcus aureus and Staphylococcus epidermis. A total number of thirty (30) students of... Continue Reading
Spondias mombin (Linn.) is a widely cultivated edible plant used in folkloric medicine for the treatment of severe infection and health disorders. This research work was carried out to isolation, purification and chemical characterization the bioactive constituents of the ethyl acetate stem bark fraction of Spondias mombin (Linn.), a medicinally... Continue Reading
The purpose of this research work is to investigate the elemental composition and antioxidant potentials of Spondias mombin (leaf, root and stem bark) at concentration of 10, 20 and 5mg/ml. using different conventional laboratory methods, also to determine the antioxidant potentials of Spondias mombin. The element present are Sodium (Na), Calcium... Continue Reading
ABSTRACT Antibiotic resistance has become a wide spread problem. The efficacy of two solvent extracts of Cymbopogon citratus against some test bacteria and fungi was evaluated. The test bacteria was; Staphylococcus aureus, and the test fungi was; Candida albicans. The phytochemical properties and the antimicrobial potency of Cymbopogon citratus... Continue Reading
ABSTRACT Antibiotic resistance has become a wide spread problem. The efficacy of two solvent extracts of Cymbopogon citratus against some test bacteria and fungi was evaluated. The test bacteria was; Staphylococcus aureus, and the test fungi was; Candida albicans. The phytochemical properties and the antimicrobial potency of Cymbopogon citratus... Continue Reading
ABSTRACT The study was conducted to assess the antibacterial activity of Phyllanthus amarus (Schum and Thonn) extract against Salmonella typhi causative agent of typhoid fever at the laboratories of the Departments of Chemistry and Theoretical and Applied Biology of the College of Science, Kwame Nkrumah University of Science and Technology,... Continue Reading
ABSTRACT The comparative evaluation of the bacterial and fungal diseases associated with Saccharum officinarum (Sugarcane) in ethnobotanical garden of Wesley University, Ondo was carried out through different microbial processes. The objectives of this work were to comparatively evaluate bacterial and fungal diseases associated with sugarcane in... Continue Reading
Morinda lucida is a tropical West African tree of medium-size, having brown colored bark, white flowers and yellowish ellipsoid seeds. It is widely used in traditional medicine systems of different countries. The preliminary phytochemical screening of different extracts prepared from bark, leaves and seeds of M. lucida revealed the presence of... Continue Reading
The plant Morinda lucida falls under the family Rubiaceae known to have wide usage in traditional medicine. Morinda lucida is a tropical West African tree of medium-size about 18–25 m tall, the bark is grey to brown in colour, flowers are white in colour, the fruit is a drupe, seed is ellipsoid, yellowish and soft. The purpose of this work is... Continue Reading
Daniella oliveri (Caesalpiniacea) is a plant found in the Amazon region and other parts of South America and Africa. The tree may reach a height of 100 feet and trunk diameter of 4 feet. Daniella oliveri (Rolfe) Fabiaceae is commonly known as Ilorin balsam (eepo-iya) or Copaihu africana . It is an indigenous African tree, found extensively in... Continue Reading
Daniella oliveri (Caesalpiniacea) is a plant found in the Amazon region and other parts of South America and Africa. The tree may reach a height of 100 feet and trunk diameter of 4 feet. Daniella oliveri (Rolfe) Fabiaceae is commonly known as Ilorin balsam (eepo-iya) or Copaihu africana . It is an indigenous African tree, found extensively in... Continue Reading
Erythrina senegalensis DC. (Coral tree) is commonly grown in West Africa as an ornamental plant and one of the oldest known African medicinal plants. The purpose of this research is to determine the antimicrobial potency, phytochemical activities, nutrients and elemental composition of the leaf and bark of Erythrina senegalensis. The organisms... Continue Reading
Morinda lucida is a tropical West African evergreen shrub or small to medium sized rainforest tree, having brown colored bark, white flowers and yellowish ellipsoid seeds. It is widely used in traditional medicine systems of different countries. This study was designed to carry out the preliminary phytochemical screening and evaluation antioxidant... Continue Reading
Bridelia ferruginea is commonly grown in Western Tropical Africa. The purpose of this research work is to determine the antimicrobial activities, qualitative and quantitative phytochemical screening, elemental composition, proximate analysis and anti-nutrient composition of purified fraction of Bridelia ferruginea extracts. The organisms used for... Continue Reading
ABSTRACT Fresh healthy carrot promote good health but harbour a wide range of contaminants. To assess the microbial quality of carrots sold in Ibadan, a total of fifteen samples were purchased from five vendors. Samples were analyzed to study the density of microorganisms by standard plate count. Bacteria belonging to eight genera were identified.... Continue Reading
ABSTRACT Fresh healthy carrot promote good health but harbour a wide range of contaminants. To assess the microbial quality of carrots sold in Ibadan, a total of fifteen samples were purchased from five vendors. Samples were analyzed to study the density of microorganisms by standard plate count. Bacteria belonging to eight genera were identified.... Continue Reading
DERMATOPHYTE are unique of fungi that effect the Keratinous tissues of lower animals and human Dermatophytoses is the medical term for fungal infection of the superficial layer of the skin, nail, hair and scalp. The prevalence study Dermatophytoses in Wuntin Dada Primary School Bauchi is to determine the prevalence of the diseases, the different... Continue Reading
DERMATOPHYTE are unique of fungi that effect the Keratinous tissues of lower animals and human Dermatophytoses is the medical term for fungal infection of the superficial layer of the skin, nail, hair and scalp. The prevalence study Dermatophytoses in Wuntin Dada Primary School Bauchi is to determine the prevalence of the diseases, the different... Continue Reading
ABSTRACT Tinea pedis, a fungal infection of the feet, especially within the toe webs and soles was studied using 40 soldiers (20men and 20women) as a case study. This infection is related to footwear having been considered to be a new dermatophytoses. The soldiers have the toe webs and soles cleaned with alcohol or sterile water and the lesions... Continue Reading
ABSTRACT Tinea pedis, a fungal infection of the feet, especially within the toe webs and soles was studied using 40 soldiers (20men and 20women) as a case study. This infection is related to footwear having been considered to be a new dermatophytoses. The soldiers have the toe webs and soles cleaned with alcohol or sterile water and the lesions... Continue Reading
ABSTRACT were carried out using soil samples collected from different locations at Osun State University, Osogbo, main campus. The soil samples were collected from twelve (12) diffeferent locations within the study site. The samples were cultured aseptically by inoculating each samples on... Continue Reading
ABSTRACT were carried out using soil samples collected from different locations at Osun State University, Osogbo, main campus. The soil samples were collected from twelve (12) diffeferent locations within the study site. The samples were cultured aseptically by inoculating each samples on... Continue Reading
ABSTRACT The isolation, identification and characterization of bacteria from organic waste were carried out. Sawdust waste (plant organic waste source) and abattoir waste water (animal organic waste source) samples were collected. The media used were nutrient agar for total aerobic plate count, McConkey agar for coliform count and cellulolytic... Continue Reading
ABSTRACT Microorganisms are essential players in the very existence of all living thing. They play a very important part in the production and biodegradation of organic food substances in which Manihot esculenta is an important player. This project evaluated the mycoflora and bacterial flora of steep cassava with the view of identifying those... Continue Reading
To determine the presence of microorganisms present in the indoor air coming from out air conditioners. CHAPTER ONE 1.0 INTRODUCTION 1.1 STATEMENT OF PROBLEM 1.2 SCOPE OF WORK 1.3 JUSTIFICATION 1.4 AIM AND OBJECTIVE CHAPTER TWO 2.0 LITERATURE REVIEW 2.1 COMMON FUNGI ISOLATED FROM INDOOR AIR 2.1.1 ASPERGILLUS SPP 2.1.2 CLADOSPORIUM SPP 2.1.3... Continue Reading
ABSTRACT The presences of pathogenic microorganism in ice cream such as Salmonella Species, Staphylococcus aureus, Escherichia coli have been well documented, but examination of pathogenic bacteria like Bacillus cereus Yersinia enterocolitica, Brucella species and E.coli O157:H7 have been rarely studied. These studies indicated... Continue Reading
ABSTRACT Thirty ripe mango fruits were pluked under sterile conditions from different geographical locations in Enugu metropolis. The mango fruits were exposed to open environment for 5 – 7 days to allow decay. The decaying mango fruits were washed with sterile water to extract the microorganisms. Using the streak plate method, the... Continue Reading
ABSTRACT The antibacterial activity of sweet orange(citrus smensin) on staphylococcus aureus and Escherichia coli isolated from would infection was studied. A total number of 100 wound sample were collected form different individual from different age range within 5-8years and examined,. The test organisms S auras and E coli were cultured... Continue Reading
ABSTRACT Microbial examination of spoilt Avocado fruits was carried out to obtained the causal organism. A total of 60 avocado from Ogbete Main market and New market Enugu were examined by culturing on nutrient agar and sabourand dextrose agar (SM), for bacteria and fungi causing post – harvest spoilage on the fruits; six bacterial species and... Continue Reading
ABSTRACT Pleurotus species are popularly known as oyster mushroom which are regarded as one of the edible mushroom of commercial important throughout the whole world. Utilization of this depends on the ability to secrete cellulolytic and hemilocellulolytic enzymes which can make use of wide spectrum of Agro industrial wastes for the growth and... Continue Reading
A total number of 60 samples of water leaf were collected from different areas in Enugu Metropolis, these samples were inoculated and examined to detect the presence of sorbitol negative Escherichia coli 0157:H7. About 35(17.5%) samples out of the 50 samples inoculated were found to be sorbitol negative E-coli. From the result E-coli 0157:H7 is... Continue Reading
Pyrexia of unknown origin (PUO) is fever that occurs for no apparent reason with failure toestablish diagnosis within one week of investigation. The fever involves temperatures ofgreater than 38.3oC which occurs sporadically for more than three weeks. Fever resolvesusually after about 3 days but can take 2 weeks. Dengue is a tropical mosquito... Continue Reading
ABSTRACT Antibacterial activity of honey obtained from two different locations in Enugu State (Nsukka & Ugwuaji) Nigeria on Staphylococcus aureus, Escherichia coli and Streptococcus pyogens isolated from wound was studied. Agar well diffusion method was used to determine the antibacterial activity of the honey on the test microorganisms. The... Continue Reading
Abstract Soaps and other cleansing agents have been around for quite a long time, a medical document from about 1500 B.C. shows that Egyptians combined animal and vegetable oils with alkaline salts to form a soap-like material used for treating skin diseases, as well as for washing and Scrubbing body or hands. The objective of this study is to... Continue Reading
ABSTRACT The antibacterial activity of three types of medicated soap on Staphylococcus aureus isolated from wound infections was conducted. Fourteen individuals with wound infections were sampled. Swab sticks were used to collect specimens from wound infections; each swab stick was streaked separately over plates of nutrient and mannitol agar and... Continue Reading
ABSTRACT Chlorella vulgaris is an edible microalgae and a highly notorious potential feed resource for many agriculturally important animal species. Chlorella vulgaris intake has also been linked to improvements in animal health and welfare. Its influence over animal development stems from its nutritive and protein-rich composition, thus leading... Continue Reading
ABSTRACT was studied. The leave was observed to be highly rich in polyphenols, flavonoid, reducing compounds and alkaloids. Glycosides, saponins and tanins were present in small amount, there was absence of Phlobotanins,... Continue Reading
ABSTRACT Phytochemical and Antimicrobial activity of the leaves of Tetrapluera tetraptera using aqueous and ethanolic extract was studied. The leave was observed to be highly rich in polyphenols, flavonoid, reducing compounds and alkaloids. Glycosides, saponins and tanins were present in small amount, there was absence of Phlobotanins,... Continue Reading
Abstract Background Newcastle disease is a highly contagious disease of birds caused by Paramyxovirus. Birds mostly affected by Paramyxovirus virus includes; chickens, turkeys, geese, ducks and guinea fowls. Newcastle disease however, remains a worldwide problem. There are three major strains of Newcastle disease virus and they are velogenic,... Continue Reading
A RESEARCH PROJECT ABSTRACT The study on the isolation and antibiogram of bacterial isolated form sealed and unsealed drugs revealed total Bacteria count ranging form 1.2 X 10 7 to 11.5 X10 8 cfu/g for unsealed and 1.5 X10 7 to 9.2 x 10 7 cfu/g for sealed. The following... Continue Reading
A RESEARCH PROJECT ABSTRACT Antibiotic sensitivity testing is the susceptibility of bacteria to antibiotics, it is carried out to determine which antibiotic will be most successful in treating a bacterial infections. The total mean colony count of the bacterial isolates in... Continue Reading
ABSTRACT In a research conducted to ascertain the antimicrobial activity of watermelon (Citrullus lanatus) bark on organisms such as Escherichia coli, Slamonella typhi and Staphylococcus aureus using the ethanol and auqous extracts of the watermelon bark applying agar-well and pour-plate sensitivity technique. It was observed that the ethanoic... Continue Reading
ABSTRACT Endophytic microorganisms have proven to be potential sources of antimicrobials against recalcitrant pathogens. Extracts of an endophytic fungus, Emericella nidulans exhibited antibacterial activity against selected pathogens. Emericella nidulans was cultivated in broth and fermented at 28 ± 2°C for 7days and 21 days to obtain bioactive... Continue Reading
Oryza sativa husks and peels of Musa paradisiaca were pretreated by hydrothermal and acid fermentation using cassava mill effluent, and co-digested in a laboratory scale reactor maintained at 45 ± 2 ⁰C for 42 days. Five digesters were used for the experiment and each digester contained equal proportion of Oryza sativa husks and Musa paradisiaca... Continue Reading
ABSTRACT Nunu is a spontaneously fermented yoghurt-like milk product consumed as a staple food commodity invirtually all parts of the Country. Its production and consumption gives way to much food security and economic benefits to the rural people. However, the process characteristics result in products which are not appealing to many people, have... Continue Reading
ABSTRACT Colocynthis citrullus (Egusi) are seeds of a creeping annual and intercropping plant made use in traditional farming practices while Irvingia gabonensis (Ogbono) are seeds of small mango-like fruit found in West Africa. The seeds when ground into paste are highly mucilaginous and are used in thickening soups. They also add to the... Continue Reading
ABSTRACT Culturally, consumption of local fermented skimmed milk (nono/tsala) is common in Northern Nigeria and many other parts of Africa and it is sometimes associated with microbial diseases due to poor hygienic practice. Hence, this study was conducted with the objectives of assessing the microbial quality and some physicochemical parameters,... Continue Reading
ABSTRACT In the for anti microbial effect the back peel of Persea americana (AVACADO) was investigated for activities . the study was to evaluate the antimicrobial efficacy of the crude ethanolic and aqueous extract of the peel of Persea americana against selected clinical isolates. The agar well diffusion method was used in study also... Continue Reading
ABSTRACT This study reviewed the importance of hand washing and health benefits derived from the practice and also highlighted the consequences of not observing strict hand washing practice. Swabs from primary school pupils showed cfu mean total viable count of 1.60 x 104, 1.50 x 104, 1.60 x 104 and 1.7 x 104 across the four schools. Salmonella... Continue Reading
ABSTRACT Milk has been known to be highly nutritive ad thus a good medium for bacteria growth and vehicles for transmission of food borne infection. A total of 16 samples of different brands of bagged milk obtained from some selected markets within Kaduna metropolis were analyzed for the occurrence of bacteria using pour plate method on selective... Continue Reading
ABSTRACT The effect of spent engine oil on soil properties and growth of Spinach (Spinacia olereacea) was investigated. Five treatments (0, 20, 40, 60, 80)ml of the spent oil were applied to soil in perforated poly bags with Spinach strands at four weeks after stabilization. Soil analysis showed that spent engine oil had no effect on both the pH... Continue Reading
Street vended foods are the major source of food borne diseases in most parts of sub-Saharan Africa including Nigeria. In this study, six samples each of six different vended foods obtained in Calabar municipality were evaluated for bacteriological quality and presence of Staphylococcus aureususing standard microbiological techniques. Each food... Continue Reading
The introduction Methodology Materials needed Characteristics of bacillus that can produce amylase isolated from Palm kernel waste water in the production of local palm oil Characteristics of bacillus specie that have the potential to bioflocculate isolated from Palm kernel waste water in the production of local palm oil Aim and objectives of the... Continue Reading
ABSTRACT The present study is to isolate fungi from rhizosphere of Moringa oleifera and screen them for the production of some very important enzymes such as amylase and protease. Twenty fungi were isolated from the soil sample from five different farms using pour plate method. The isolates were then identified based on their colonial morphology... Continue Reading
1.0 INTRODUCTION AND LITERATURE REVIEW Ear wax is normally produced in the ear canal. It is made up of the secretions of specialized sets of glands located in the ear canal. Sebaceous glands secrete sebum. Sebum is mostly a combination of fatty acids. Modified apocrine sweat glands release a secretion that combines with the sebum to form... Continue Reading
ABSTRACT In the for anti microbial effect the back peel of Persea americana (AVACADO) was investigated for activities . the study was to evaluate the antimicrobial efficacy of the crude ethanolic and aqueous extract of the peel of Persea americana against selected clinical isolates. The agar well diffusion method was used in study also... Continue Reading
The study was carried out to investigate the effect of aqueous and petroleum ether extract of momordica charantia on blood glucose level of albino rats.The extraction was done using petroleum ether and water .Thirty two albino rats were grouped into eight (A ,B,C,D,E,F,G,H). Comprising of four in each group .Diabetes was induced with a single Dose... Continue Reading
ABSTRACT Honey has since been found to possess antibacterial property and is therefore employed for wound and gastro-intestinal disease therapy. This study evaluates the antibacterial spectrum and efficacy of honey and compared with tetracycline. Different concentrations (50, 75 and 100.0 %) of honey were studied in-vitro using Staphylococcus... Continue Reading
ABSTRACT The castor plant is a robust perennial shrub of Euphorbiacaea family. The seeds are documented to have an antibacterial profile against some bacterial organisms such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus vulgaris, and Streptococcus pyogenes. This study was carried out to... Continue Reading
1.0 INTRODUCTION 1.1 BACKGROUND OF STUDY Schistosomiasis is a chronic human disease caused by parasitic flatworms called schistosomes or blood flukes. The schistosomes live i1n the blood vessels associated with the intestine and urinary bladd1er where they reproduce sexually. Schistosomiasis is one of the most wide spread parasitic disease that... Continue Reading
ABSTRACT This study was carried out to investigate the nutritional value and the antibacterial activity of bitter kola, (Garcinia kola). The result of the proximate analysis shows that bitter kola contains 51.95% moisture, 5.55% Ash, 9.75% crude fibers, 4.65% crude fat, 7.35% crude protein and 20.85% carbohydrate. The anti-bacterial activity shows... Continue Reading
INTRODUCTION 1.1 Background of study Juice is a liquid that is naturally contained in fruit and vegetables. It can refer to liquid that are flavoured with these or other biological food resources such as a beverage or used as an ingredient or flavouring in food, (Lewis, 2002), fruit juice is produce by squeezing or crushing fruit. Many... Continue Reading
ABSTRACT Gastro Intestinal Helminthiasis was investigated among primary school children in Ihengwu village, Oko between February and March 2015. Sixty samples of freshly collected faeces from sixty randomly selected primary school children between the age of four and ten years from two selected primary schools; Gaius Benton and Pope John primary... Continue Reading
ABSTRACT This study was conducted to examine the fungi present in spoilt onion bulb. Five different sample of spoilt onion bulb were blended together and suspended in sterilized distilled water which was used to inoculate Sabouraud dextrose agar (SDA). Four different fungi were isolated from the sample and the morphological characteristics shows... Continue Reading
Abstract The ectoparasitic infestation of free range domestic fowls were surveyed between August and November, 2014. A total of 105 ectoparasites belonging to four species of two genera were collected. These were made up of three lice and one ixodid tick. The percentage occurrence of these parasites are: Menacanthus stramineus (50.5%),... Continue Reading
ABSTRACT Rotavirus is a common cause of diarrhea in children. This study was carried out to determine the prevalence of rotavirus antigen in children below five years presenting with diarrhea in Gombe State. Thirty (30) stool samples were analysed using ELISA KIT. Out of the total number of samples analyzed, a prevalence of 6.67% was recorded... Continue Reading
ABSTRACT Milk, Yoghurt and Cheese are highly valuable food which are readily digested and have high concentration of nutrients which have proved to be a heaven of microbes, studies were conducted on the isolation and identification of microbes [Fungi and Bacteria] on peak canned milk, yoghurt, locally prepared cheese and cheese water. It was... Continue Reading
Nine samples of freshly prepared kunu was taken from local vendors and hawkers at Tanke area, Ilorin, Kara State and analyzed for microbial quality. The pH of the samples ranged from 2.66 to 4.06. The total bacterial count ranged from 1.5×104 to 7.4×104 cfu/ml; the total coliform count ranged from 1.0x102 to 8.0x103cfu/ml; the faecal coliform... Continue Reading
Bacteria are group of microorganisms all of which lack a distinct nuclear membrane (and hence are considered more primitive than animal and plant cells) and of most which have a cell wall of unique composition. Most bacteria are unicellular; the cells maybe spherical (coccu), rod-shaped (bacillus), spiral (spirillum), comma-shaped (vibrio) or... Continue Reading
ABSTRACT Infection with Chikungunya virus (CHIKV) is highly infectious disease that causes some dermatological manifestations and clinical symptoms. CHIKV is a member of the family Togaviridae. Structurally, it has an encapsidated genome composed of single-stranded RNA. This study is carried out to determine the seroprevalence and prevalence of... Continue Reading
ABSTRACT . Microbial quality of commercially prepared yoghurt sold in umuahia was evaluated. Ten(10) different brands of Yoghurt was purchased randomly from hawkers and beverage stores in Umuahia urban and analyzed in the laboratory. Six (6) of the samples where registered by NAFDAC [ Sample A, B, C, D, E and F,] while four(4) others were not... Continue Reading
-the aim of this study is to carried out the Antimicrobial and phytochemical effect on five medicinal plants used as chewing sticks in Owo local government of Ondo State, Nigeria. Methods- The methods used for this study are antimicrobial assay, and phytochemical screening. The aqueous, ethanol and ethyl acetate extracts of the ... Continue Reading
ABSTRACT The study determines the antifungal effects of aqueous, ethanolic and methanolic extracts of Garcinia kola on some selected fungal isolates and their phytochemical constituents. The antifungal sensitivity and Minimum Inhibitory Concentration (MIC) were determined by agar well diffusion and agar dilution methods, respectively using... Continue Reading
Abstract Objectives: The present study reported the antibiotic susceptibility profile and pattern of pathogens isolated from in- and out-patients with respiratory tracts infections. Methods: Sputum and throat swab samples were collected aseptically from subjects and cultured on the appropriate bacteriological media. Bacterial isolates were... Continue Reading
ABSTRACT A total of 100 subjects were screened for S. aureus strains, thirty (30) S. aureus isolates were obatained. The carriage of β-lactamase among the 30 isolates was determined by using starch iodine agar plate method. It was found that 19(63.3%) of the S. aureus strains were β-lactamase producing. Plasmid curing of the β-lactamase... Continue Reading
Studies of plant promoting growth of bacillus it has been shown that bacillus methylothropicus enhance the growth of varies plant such capsicum annum... Continue Reading
ABSTRACT Antimicrobial activity of ethanolic extract of Mangifera indica peel was tested on some pathogenic organisms. The extract was tested for activity against, Baciollus subtilis, Staphylococcus aureus, Escherichia coli, Salmonela typhii, Pseudomonas notatum and Aspergillus fumigates. The extract exhibited activity against the organisms in... Continue Reading
Mutagenesis Lab Procedure 1. Clean your hands and work area. • Clean your hands with hand sanitizer • Put all the food away 2. Acquire a Petri dish from your instructor and label it using a waterproof marker. • Write your name in small letters around the outside edge of the bottom of the dish. • Draw lines on the bottom of the dish to... Continue Reading
ABSTRACT Microorganisms are important components in the structure and function of organic matter in soil, hence this work was done to identify bacterial communities present in flooded soil. Soil samples were collected from eight different areas within Benin metropolis, Edo State, Nigeria. The samples were cultured on using pour plate... Continue Reading
ABSTRACT This study was carried to with the aim of determining the antibacterial effect of Calotropis procera against some bacteria isolated spoilt nunu. Bacteria isolated from spoilt nunu were identified based on their Gram characteristics, morphology and biochemical characteristics. Calotropis procera was extracted using ethanol, methanol and... Continue Reading
ABSTRACT The microbiological assessment of Garri, sold in two different markets in Benin City metropolis, was carried out using the pour plate technique. The mean total count ranged from 15.0 to 29.4 × 104 cfu/g in Oba market and Santana market respectively. A total of six organisms were identified in Santana Market samples while a total of ten... Continue Reading
ABSTRACT 40 nasal samples were collected from apparently healthy males students at Eguare Secondary School, Ekpoma, whose ages fell within the ranges of 15-20 years were selected and screened for Staphylococcus aureus and Candida albicans using Mannitol Salt Agar and Sabouraud dextrose agar respectively. A total of 15 (37.5%) samples were positive... Continue Reading
ABSTRACT Hepatitis C virus (HCV) is a meticulous factor of liver disease and one of the most important health issues worldwide. Hepatitis is the inflammation of the liver produced by autoimmune disease, alcohol or drug abuse, genetic disorder or microbial infection. To detect the presence; prevalence; incidence; and the relationship between the... Continue Reading
ABSTRACT A study was undertaken to determine typical concentration of airborne bacteria and fungi (microflora) in Enugu State University of Science and Technology (ESUT) in the tropical rainforest environment of Nigeria. Aerial sampling was conducted at various faculties and departments each day. The air samples were collected thrice daily.... Continue Reading
ABSTRACT The study assessed the prevalence, intensity and environmental risk factors affecting the transmission of soil transmitted helminthiasis in eleven randomly selected communities in Nsukka and Uzouwani Local Government Areas of Enugu State, Nigeria. It also determined the effect of peoples’ knowledge of mode of transmission of the... Continue Reading
ABSTRACT The study involved the isolation of (2) isolates titled RMN1, RMN2, belonging to the Genus Serratia from ( 2) soil samples collected from the following two strategic locations in university of Abuja, permanent site: Termite hill besides Girls hostel dump ground and from Faculty of Agricultural Science farm land. The isolates were... Continue Reading
ABSTRACT This research work involve the isolation of two isolates titled RMN1 and RMN2, belonging to the Genus Serratia from two soil samples collected from the following two strategic locations in university of Abuja, permanent site: Termite hill besides Girls hostel dump ground and from Faculty of Agricultural Science farm land. The isolates... Continue Reading
INTRODUCTION Dairy products are various products derived from cow’s milk or that of other female mammals such as goat, sheep, yaks, horses, camel. Dairy products include yoghurt, nono (fermented cow’s milk, madara (unfermented cow’s milk, cheese, whey, condensed and evaporated milk.) (cultureforhealth, 2015). Yoghurt, nono, Madara has become... Continue Reading
Abstract Abstract Background: Occult hepatitis B infection (OBI) is defined as serologically undetectable hepatitis B surface antigen (HBsAg-ve), despite the presence of circulating HBV DNA (Allain, 2005). It has been documented that one of the frequent complications of HIV infection is hepatitis B co-infection and due to the common methods of... Continue Reading
ABSTRACT The microbiological and physicochemical characteristics of soil receiving palm oil processing waste (POPW) in Abraka were investigated. The effect of palm oil processing waste was tested on loamy, sandy and clay soils. The pour plate technique was used for the isolation of the organisms. The total mean bacterial count ranged from... Continue Reading
ABSTRACT Safety of food is a basic requirement of food quality. A total of 25 street food samples (Jollof rice, egwusi soup, ugu, water leaf and green), were randomly purchased from five different vendors in Ogbete main market Enugu. The samples were transported in ice to the laboratory. The samples were bacteriologically analyzed using pour plate... Continue Reading
ABSTRACT A total of 50 blood (30 pregnant and 20 HIV) samples were obtained from pregnant women and PLWHA attending clinic at UNTH Ituku-ozalla. Samples were screened for hepatitis c viral infection using the rapid one step hepatitis C virus test strip. Two (6.66%) pregnant women were positive and four (20%) HIV patients were positive for... Continue Reading
ABSTRACT Effect of different carbon sources on the growth of antimicrobial producing Bacillus species in ogiri-egusi sold in Ogbeta, Orie, Artisian, Kenyatta, Enugu State were evaluated using standard microbiological and analytical methods. 12 bacteria were isolated and identified such as Bacillus subtilis,(33.33), Bacillus pumulis (41.66),... Continue Reading
ABSTRACT Comparative examination of three main sources of water supply in Independence layout Enugu was carried out with a view to determine their levels of contamination of bacteria. The sources of water examined are tap water, well water and stream water. The pour plate method was used to examine the water samples. Bacteria isolated from the... Continue Reading
ABSTRACT A major challenge for achieving successful mosquito control is overcoming insecticide resistance. Bacillus thuringiensis which is one of the most effective biolarvacide for control of species of mosquitoes and monitoring of larval susceptibility is essential to avoid resistance development. Mosquito larvacidal activity of Bacillus... Continue Reading
ABSTRACT Antibacterial activity of honey obtained from two different locations in Enugu State (Nsukka & Ugwuaji) Nigeria on Staphylococcus aureus, Escherichia coli and Streptococcus pyogens isolated from wound was studied. Agar well diffusion method was used to determine the antibacterial activity of the honey on the test microorganisms. The... Continue Reading
Abstract The main purpose of this work is to investigate and compare the antimicrobial properties of Chrysophyllum albidum seed and stem bark essential oils, against some selected pathogenic isolates, to compare the phytochemical composition of the oil of Chrysophyllum albidum seeds and stem bark, to analyze the chemical compounds responsible for... Continue Reading
Abstract The purpose of this present study is to determine the antibacterial potency of essential oil of Polyalthialongifolia, to determine the presence of phytochemicals and structural determination of bioactive component from the leaf portion using GCMS In this present study, the antibacterial activity from the essential oils of... Continue Reading
Abstract This study aims to determine the phytochemical characteristics, structural determination of bioactive component from the leaf extract using GCMs and experimentally investigation of the antibacterial activity of essential oil from Persea americana and Citrus sinensis leaf against seven bacteria (Gram-positive and Gram-negative) which are... Continue Reading
ABSTRACT In African folk medicine, water infusion of Mangifera indica stem bark are highly valued in thelocal treatment of malaria fever, diarrohea, diabetes mellitus and liver diseases. Various part ofthis plant can also be used in treatment of toothache, dysentery, anemia, hypertension andinsomnia in different parts of the world. This study was... Continue Reading
ABSTRACT The present experiment aims at investigating the growth behaviour of different yeast strains inselected carbon, nitrogen and stress environment to obtain strains with prospects for industrialapplication. Specifically, the study is set to: isolate yeast from decaying mango fruit ... Continue Reading
Microbiological tests revealed that Hibiscus sabdariffa plant extract has antibacterial properties thus verifying folklore medicine in the treatment of abscesses, bilious conditions, cough, dysuria and scurvy (Morton, 1987; Perry, 1980 Ross, 2003). The extraction type of aqueous ... Continue Reading
ABSTRACT Mushroom has been an important food to man for millennia. Several species of Pleurotus has been cultivated successfully on different substrates including Palm bunch, Saw dust and Corn cob. Pleurotus pulmonarius has also been cultivated but there is a gap in knowledge as to the effect of different substrates on the phenotypic, nutritional,... Continue Reading
ABSTRACT The antibiotic susceptibility pattern of six Gram negative pathogenic bacteria; Escherichia coli, Pseudomonas, Klebsiella, Salmonella, Shigella and Enterococcus was evaluated. The test organisms were subjected to antibiotic susceptibility test using the Kirby Bauer method. A multi-disc containing eight (8) antibiotics; Ceftrazidime... Continue Reading
This project work is aimed at investigating whether there is a relationship between weight and blood pressure of patients with high blood pressure. Background of the Study The number of people with high blood pressure is in the increase and research has shown that this condition is heightened by overweight of the patient. That is to say, the more... Continue Reading
Yoghurt is a cultural diary product produced by lactic fermentation of milk (Hui, 1992). Yoghurt is produced by the controlled fermentation of milk by two species of bacteria; Lactobacillus bulgaricus and Strptococcus thermophilus. These two species of bacteriae have now been established as the yoghurt starters. Any sort of milk may be used to... Continue Reading
ABSTRACT Newcastle disease (ND) is one of the most important avian viral diseases because of its high economic impact on the poultry industry. Newcastle disease is now claimed to be responsible for 70-80% of annual death in village chickens. Losses in hundreds of poultry birds of various ages and sexes in Anyigba Kogi State of Nigeria is... Continue Reading
ABSTRACT The prevalence of Trichomonas Vaginalis among adult in \'Osumenyi\' in Nnewi south local government Area of Anambra state was carried out. This was done using a stotal of one hundred adults from there different clinics viz ;- Health center, family planning and Antenatal Clinic in Osumenyi; in Nnewi South L.G.A of Anambra State.... Continue Reading
ABSTRACT This work is aimed at determining the incidence of bacterial injections such as Salmonella and Escherichia coli which cause health hazards to the poultry in their feeds. Sample feed were obtained from commercial mash operation/ pellet mill operation at New heaven and top feed at Ogbete main market Enugu. The samples were screened for... Continue Reading
ABSTRACT In thus work the incidence of candidacies was studied using selected patients of university of Nigeria teaching hospital Enugu analyzed for the presence of candidacies yeast infection. The specimens were cultured on seaboards agar and blood agar and subculture isolated were C aibicans C Tropical C Knusei C Pseudoizo P cals C paraknusei... Continue Reading
ABSTRACT In the work, samples of Garri obtained from Ogbete market were evaluated for microbial contamination. Four sample of Garri from different sources of supple were collected from Ogbete main market Enugu Okwo, Nkalagu, Emene, and Abakaliki respectively. The sample were extracted using equal volume of sterile distilled water and serially... Continue Reading
ABSTRACT The Anatomy of this project write-up encompasses the ordinary look of enzymes as organic molecules, rather it sheds more light into the comprehensive view and determination of the catalytic and specific activity enzymes particularly- DIASTASE, MALTASE and ZYMASE can exploit in industrial application, also critically important is the... Continue Reading
ABSTRACT Onion is associated with micro-organisms which are capable of causing spoilage. Onions with rotwere examined microscopically. A solution of it was made and the serial dilution of 1:10-1 to 1:1-5 were made from 10ml of the onions sample. From the dilution, 1ml of each diluent was plated on nutrient and saboor and dextrose agree to permit... Continue Reading
ABSTRACT This research work was on maize (Zea may) abject in sorghum vulgare) beer brewing the sorghum was malted and milled. Malting was achieved by sleeping the grains in water at 280c for 48 germination of the grain was allowed for 72 hrs and kilmed in air oven at 550c for 48 hrs. maize as an adjuct was in corporate into the malted milted... Continue Reading
ABSTRACT The isolation and identification of bacteria associated with 150ml sepsis studies were carried out to 250 patient at National Orthopedic Hospital Enugu. (250) Two hundred and fifty patients with 150ml sepstis was grouped in to in patients and out patients. The sample collected are wound pus and wound exudates which were collected from... Continue Reading
ABSTRACT The microbial changes which took place during the steeping and storage of pan (ogi) was studied. The steeped water had an initial PH of 6.8 which latter reduced to 4.9 at the end of steeping. The bacterial number for the steeped water ranged from 4.7x104 to 3.2 x 107 cfu/ml while the fungal number ranged from 1.5 x103 to 5.7 x 106 cfu/ml.... Continue Reading
ABSTRACT This project work is carried at the isolation and characterization of pathogenic bacteria from hawked suya meat, were bought in full from the sellers and was collected in raps of aluminum foil. It was then taken to the laboratory for analysis the samples were homogenized and serial dilution of the sample was made out using 5 ml sterile... Continue Reading
ABSTRACT A total of fifty faecal samples were collected form pupils in airport primary school Emene Enugu were used for the study. The duration of the investigation was between February August 2005. These samples were cultured and their causative organisms determined using macconkey agar, deocycholate citrate agar and an alkaline peptone water... Continue Reading
ABSTRACT This project work which is all about the fungal infestation of bakery product BREAD, explain the kind or type of fungal organisms that are responsible for the spoilage on contamination of bread. The infestation of fungal organism on bread depends on the kind and number of the agent present and upon the environment about them. this... Continue Reading
ABSTRACT The incidence of the infestation of plasmodium species (P.Falciparum, P. Vivax, P.Ovale, and P. alaria) which causes human malaria in Enugu metropolis was conducted at Parklane Hospital using their patients. Thick and thin, smear of blood were made and examined after proper staining. The staining method which proved effective were the... Continue Reading
ABSTRACT A study was carried to ascertain the potency of some techniques used in diagnosis of Human Immunodeficiency virus (HIV). Two hundred and five samples (203) were collected from the patients from the university of Nigerian teaching hospital and was analysed using Elisa kit, which has the ability to detect antibodies and antigens in the... Continue Reading
ABSTRACT Samples of water from the different hostels in campus II and other source of water production namely Ajali-owa water, from bore hole 9th mile and spring water from GRA water board were water collecterd and analysed using total plate count and multiple tube fermentaton techniques coliform. The results shows no coliforms in production water... Continue Reading
ABSTRACT In comparing the antimicrobial strength of three most common antibiotics, which includes: streptomycin chloranphenicol and gentarccin. Samples of urine and high vaginal swab (H.V.S) were collected from park lane Enugu. The organisms isolated were pure culture of staphylococcus aureus and Escherichia coli Sensitivity test was carried out... Continue Reading
ABSTRACT The study on anaemia in pregnancy is aimed at determining packed cell volume (PCV), Haemoglobin (Hb) level and Erythrocyte sedimentation rate (ESR) of pregnant women, as well as the selferity, significance of maternal, age, educational level, occupation and gestational age to the occurrence of anaemia in pregnancy managed, diagnosed and... Continue Reading
ABSTRACT The survey of urinary sehistomiass in Ishele in Amagunze, Nkanu East local Government was carried out using two methods of diagnosis i.e combination of questionnaire on clinical signs and symptoms, and laboratory microscopy using primary school children in the area as test samples. A total of fifty samples were randomly collected reveals... Continue Reading
ABSTRACT This research work was carried out to examine the physicochemical and phytochemical constituents of honey and Shea butter samples respectively and their antibacterial effect on Staphylococcus aureus and Klebsiella pneumoniae . The physicochemical screening of honey showed that the honey samples used have low water activity, low moisture... Continue Reading
TABLE OF CONTENT CONTENT TITLE PAGE CERTIFICATION DEDICATION ACKNOWLEDGEMENT ABSTRACT TABLE OF CONTENTS CHAPTER ONE 1.0 PREAMBLE 1.1 INTRODUCTION 1.2 OBJECTIVE OF STUDY 1.3 SCOPE OF WORK CHAPTER TWO 2.0 LITERATURE REVIEW 2.1 FATS AND OILS 2.2.0 COMPONENTS OF MUCUNA SEEDS 2.2.1 NUTRITIONAL PROPERTIES 2.2.2 ANTINUTRITIONAL PROPERTIES 2.2.3... Continue Reading
ABSTRACT The extraction of alkaloid from Gaxcina kola (bitter Kola), Kola acuminated (Oji Igbo) and Kola Vera (Oji Hausa) were carried out using 10% enthanoic acid and 10% ethanol to separate the alkaloid from the residue. The alkaloid was extracted using 5% ammonium hydroxide. From the sample results obtained on alkaloid for the sample 9.16% oji... Continue Reading
TABLE OF CONTENTS CHAPTER ONE 1.0 INTRODUCTION. 1.1 AIMS AND OBJECTIVES CHATER TWO 2.0 DESCRIPTION OF YEAST 2.1 SCIENTICCLASSIFICATION OF YEAST 2.2 HISTORY OF YEAST 2.3 GROWTH 2.4 ECOLOGY 2.5 REPRODUCTUION 2.6 USES OF YEAST 2.6.1 ALCOHOLIC BEVERAGE 2.6.2 DESTILLED ALCOHOL 2.7 GENERAL DESCRIPTION & APPLICATION OF GALACTARIC ACID 2.8 CHEMISTRY... Continue Reading
TABLE OF CONTENTS CHAPTER ONE 1.0 INTRODUCTION CHAPTER TWO LITERATURE REVIEW 2.0. SENA TORA 2.1.1 TAXONOMY OF SENNA TORA [NATURE SERVE, 2007] 2.1.2 GEOGRAPHICAL DISTRIBUTION OF SENNA TORA 2.1.3 GROWTH REQUIREMENTS OF SENNA TORA 2.1.4 PROPAGATION OF SENNA TORA 2.1.5 IMPORTANCE USESOF SENNA TORA 2.2. LIVER 2.2.1 ANATOMY OF THE LIVER 2.2.2 STRUCTURE... Continue Reading
ABSTRACT Presence of Escherichia coli from water supplied by water tanker suppliers in Enugu City was determined. Out of eight different water tanker supplies examined which were designated alphabetically as A, B, C, D, E, F, and H. Escherichia coli was isolated from four tanker suppliers. The membrane filtration technique was used and the... Continue Reading
TABLE OF CONTENTS CHAPTER ONE 1.0 INTRODUCTION CHAPTER TWO 2.0 LITERATURE REVIEW CHAPTER THREE 3.0 MATERIALS AND METHODS 3.1 SAMPLE COLLECTION 3.1.1 STERILIZATION OF MATERIALS 3.1.2 PREPARATION OF ERYTHROCYTE FOR HAEMAGGLUTINATION INHIBITION TEST (CHICKEN RED BLOOD CELL) 3.1.3 HAEMAGGLUTINATION ASSAY FOR (STOCK ANTIGEN) 3.1.4 HAEMAGGLUTINATION... Continue Reading
TABLE OF CONTENTS CHAPTER ONE INTRODUCTION CHAPTER TWO LITERATURE REVIEW DEVELOPMENTAL CYCLE OF CHLAMYDIA MORTALITY/MORBIDITY CHLAMYDIAL URETHRITIS MODE OF TRANSMISSION EPIDEMIOLOGY AND PATHOGENESIS DIAGNOSIS PREVENTION AND CONTROL TREATMENT/THERAPY CHAPTER THREE MATERIALS AND METHODS MATERIALS CHAPTER FOUR RESULTS CHAPTER FIVE DISCUSSION,... Continue Reading
ABSTRACT Bacteria contamination on Nigerian currency evaluated by exposure method. Pure colony of each isolates were identified using colonial morphological characteristics, as well as biochemical reaction. Nutrient agar, blood agar and sabouraud dextrose agar were exposed for 5 minutes in various Nigerian currency mention in chapter 3. Seven... Continue Reading
TABLE OF CONTENTS CHAPTER ONE 1.0 INTRODUCTION CHAPTER TWO 2.0 LITERATURE REVIEW 2.1 FERMENTED FOODS 2.2 Pentaclethra Macrophylla 2.3 Botany 2.4 ECOLOGY 2.5 Growth and Development 2.6 Method of Production 2.6.1 Microorganism involved in ugba fermentation 2.6.2 Ugba... Continue Reading
TABLE OF CONTENT COVER PAGE DEDICATION ACKNOWLEDGMENT CHAPTER ONE: GENETICS GENE STRUCTURE OF A GENE TRANSCRIPTION TRANSLATION DNA REPLICATION AND INHERITANCE CHAPTER TWO: BENEFITS OF GENETICS GENETIC TECHNOLOGY¬¬ BENEFITS ON INDUSTRIAL BENEFIT ON AGRICULTURE BENEFIT ON MADICINAL GENETIC ENGINEERING CHAPTER THREE: HARMFUL OF GENETICS... Continue Reading
ABSTRACT A total of 400 urine samples were collected between January-August, 2010 among residents of Emene, Ujodo L.G.D.C of Enugu State. The samples were analysed using standard method- macroscopy for the presence of blood and microscopy for the the ova of schistosomes. Results revealed that 5 of them had the infection- 4(1.0%) and 1(0.25%)... Continue Reading
ABSTRACT Locally brewed beer (sorghum beer) is an indigenous alcohol average. It is produced mainly from sorghum grain (Sorghum vulgare ) and the process of brewing involves malting, mashing, souring, fermentation and packaging. Locally brewed beer without hops has a short shelf-life. The aqueous extract of a tropical plant, bitter-leaf was used... Continue Reading
ABSTRACT Potassium bromate is an additive widely employed by bread makers to improve bread quality. On account of its deleterious effect and carcinogenicity in humans, certain levels of potassium bromate are not allowed in bread. Use of potassium bromate in bread is banned in many countries including Nigeria. The present evaluation was carried out... Continue Reading
TABLE OF CONTENTS CHAPTER ONE 1.0 INTRODUCTION CHAPTER TWO 2.0 LITERATURE REVIEW 2.1 PRODUCT DESCRIPTION 2.2 PRODUCTION OF YOGHURT MATERIALS 2.3 VARIETIES IN YOGHURT PRESENTATION 2.4 HEALTH BENEFITS OF YOGHURT 2.5 NUTRITIONAL PROFILE OF YOGHURT 2.6 FACTOR THAT ALTER THE QUALITY OF YOGHURT 2.7 ... Continue Reading
ABSTRACT Samples of well water were collected from four (4) different wells located in different parts of New Haven in Enugu urban area, for bacteriological analysis. Total bacterial counts, using pour plate method were carried out. Nutrient agar plates were used for general bacterial count, while MacConkey agar plates were used for... Continue Reading
ABSTRACT This work was carried out to investigate the chemical properties of the water extract of zobo (Hibiscus sabdariffa) drink using the modern atomic absorption spectrophotometer. Zobo drink is a popular drink in the Nigeria, especially in the northern part of Nigeria. The result of the analysis indicated that the drink is high in vital... Continue Reading
INTRODUCTION Chlamydiae are small gram-negative obligate intracellular microorganisms that preferentially infect squamocolumnar epithelial cell (www. Google. Com.2010). Chlamydia is the most prevalent sexually transmitted disease resulting in urethritis, with frequencies exceeding those of Neisseria gonorrhoeae. In the United State for instance,... Continue Reading
ABSTRACT Ethanol, Bleach and Phenolics are three kinds of disinfectants which have been widely used in common laboratories. In this study, a compared experiment on these three disinfectants efficiency was conducted against Staphylococcus aureus and Pseudomonas aeruginosa using agar hole diffusion method. Different concentrations of bleach (1%, 2%,... Continue Reading
ABSTRACT Three types of sachet water samples and three types of borehole water samples all from Owerri Municipal, Imo State were collected and analyzed for physicochemical parameters. A total of twenty (21) parameters including Odour, Colour, pH, Conductivity, Acidity, Alkalinity, Total Solids, Dissolved Solids, Suspended Solids, Dissolved Oxygen... Continue Reading
TABLE OF CONTENT CHAPTER ONE 1.0 INTRODUCTION 1.1 AIMS AND OBJECTIVES OF THE RESEARCH CHAPTER TWO 2.0 LITERATURE REVIEW 2.1 SCIENTIFIC CLASSIFICATION OF SENNA TORA 2.2 DESCRIPTION OF SENNA TORA 2.3 SOME COMMON NAMES OF SENNA TORA 2.4 GEOGRAPHICAL DISTRIBUTION OF SENNA TORA 2.5 GROWTH REQUIREMENTS OF SENNA TORA 2.6 PROPAGATION OF SENNA TORA 2.7... Continue Reading
ABSTRACT This work evaluates the antioxidant potential of Monodora myristica (African nutmeg). Monodora myristica extract was obtained by solvent extraction using n-hexane and used as treatment on freshly prepared crude palm kernel oil and palm oil. Equal volume of oil samples were subjected to different concentration of extract treatment... Continue Reading
TABLE OF CONTENTS CHAPTER ONE INTRODUCTION 1.1 Herbs 1.2 Objectives of study CHAPTER TWO LITERATURE REVIEW 2.1 History of Piper methysticum (Awa) 2.1.1 Chemistry of Awa 2.1.2 Uses of Piper methysticum 2.1.3 Effect of Awa on the fungal growth 2.1.4 Hepatotoxicity of Piper methysticum 2.1.5 Phytochemicals of Piper methysticum CHAPTER THREE MATERIALS... Continue Reading
ABSTRACT The strategy of utilizing mechanochemical synthesis to obtain metal-organic frameworks with high surface area and nanodisperse particles has been espoused. Transition metal ions have been shown to form complexes with organic moieties such as benzyl-N,N-dimethyldithiocarbamate. It has been established that iron in its oxidised state (Fe 3+... Continue Reading
ABSTRACT This research work was carried out to examine the physicochemical and phytochemical constituents of honey and Shea butter samples respectively and their antibacterial effect on Staphylococcus aureus and Klebsiella pneumoniae . The physicochemical screening of honey showed that the honey samples used have low water activity, low moisture... Continue Reading
ABSTRACT The effect of pesticides, chchlorodipheny trichloroethane (DDY) and Benzene hexaxhoride (BHc) on the microflora of the three types of soil (loamy, clay and sandy soil) was conducted. The serial dietitian technique was employed up to 10-3 using nutrient agar, potato dextrose agar and sabourand agar plates. The isolation of both bacterial... Continue Reading
ABSTRACT The antibacterial activity of three type of medicate soap on staphylococcus aureus isolated from wound infections was conducted. Fifty individual with would infection within the age range of 9-73 years were sampled. Swabsticks were used to collect specimens from wound infection. each swabstick was streaked separately over plates of... Continue Reading
ABSTRACT The prevalence of streptococcus pneumonia in pneumonia patients using university of Nigeria Teaching Hospital (UNTH) Enugu as a case study was carried out based on the fact of the pneumonia is one of the commonest afflictions of the aged people and children worldwide. A total of 50 samples were collected. 12 (24%) of the sample were from... Continue Reading
ABSTRACT Isolation and characterisatics of an antibiotics producing bacterium collected from the soil near the arbatior in artesan market Enugu was achieved by characterizing a collection of 74 soil bacteria, including eubacteria and actinomycetes, according to their ability to produce antibacterial antibiotics and their growth responses to 43... Continue Reading
ABSTRACT The purpose of this research was to assess the hygienic properties of commercially available dairy foods especially fresh/raw milk. Milk, when synthesized in the udder of a healthy cow is virtually sterile. As milk passes through the teat cistern and teat channel, it may be contaminated with low levels of bacteria (<1000/ml), which are... Continue Reading
ABSTRACT Microbial evaluation of twenty samples of raw milk from a diary farm (Emene fulani cattle rearers) was carried out using five method: viz direct microcopies count nutrient agar count, Blood agar count, Mac conkey agar count (celiforms only) and Acid fast bacilli staring was done to assay for the presence of the Tubercle bacillus. The... Continue Reading
ABSTRACT Ugba (Ukpaka) is the Ibo name of the fermented African Oilbean seeds (Pentaclethra macrophylla, Benth). It is a popular traditional food condiment in Nigeria especially among Ibo ethnic group generally. It is produced by natural (local) fermentation in homes as a small family business. It is an important and cheap source of protein for... Continue Reading
ABSTRACT Juice was extracted from banana (Musa sapientum) pulp with the addition of lemon juice and was inoculated with Baker’s yeast (Saccharomyces cerevisiae) and held at 30 for seven days. The result of the yeast count increases at 48hr, and at 96hr the yeast count decreased gradually. It ranges from 4.9x107 cfu/ml at 0hr, 5.1x107 at the... Continue Reading
ABSTRACT Twenty (20) samples of suya meat in Enugu were collected randomly and analyzed microbiologically and the isolates were identified as Staphylococcus aureu (35%), Escherichia coli (15%), Streptococcus species (15%), Pseudomonas (35%). The most frequently isolated organisms were Staphylococcus and Pseudomonas. The total viable bacterial... Continue Reading
ABSTRACT The bacterial contaminants associated with commercial poultry feeds from three companies in Nigeria (vital, Guinea and Top) were studied using streak plate techniques. The culture media used were Nutrient agar and Mac Cokey agar. The aim/ objective of the study is: To ascertain the microbial safety of commercial poultry feeds produced by... Continue Reading
OF CARITAS UNIVERSITY STUDENTS ABSTRACT In order to access the prevalence and sensitivity pattern of urinary pathogens, 60 midstream urine samples from students of Caritas University were investigated using cultural methods. Samples were examined microscopically and... Continue Reading
ABSTRACT The susceptibilities of Salmonella typhi and other pathogens to antibiotics and hot aqueous extract of Hibiscus sabdariffa were investigated using agar diffusion and agar well diffusion methods respectively. Salmonella typhi was sensitive to ampicillin, cetriaxone, ciprofloxacin, gentamycin of ofloxacin and perfloxacin.... Continue Reading
ABSTRACT Stored locust bean samples were purchased from Uselu market and analysed for their fungal load and physicochemical properties such as moisture content, pH value and titratable acidity. Enumeration of the total fungal species was carried out by pour plate technique using appropriate medium, potato dextrose agar (PDA) with antimicrobial... Continue Reading
CHAPTER ONE 1.0 INTRODUCTION Crude oils are composed of mixtures of paraffin, alicylic and aromatic hydrocarbons. Microbial communities exposed to hydrocarbons become adapted, exhibiting selective enrichment and genetic changes resulting in increased proportions of hydrocarbon-degrading bacteria and bacterial plasmids... Continue Reading
INTRODUCTION The seed of locust beans ( Parkiabiglobosa ) plant found growing in the Savannah Africa provides one of the popular seasonings in African diet. The nutritious and delicious food spice is popularly called “ogiri” in Igbo, “iru” in Yoruba and “dawadawa” in Hausa in Nigeria. It is heavily consumed in Nigeria, Ghana, Sierre... Continue Reading
ABSTRACT The transmission of infection via fomites constitutes a major threat to public health especially in the developing countries. This study was carried out to investigate the microbial carriage and antibiotics pattern of bacteria on the door handles of campus shuttle operating in the Federal University of Technology Akure. Samples were... Continue Reading
ABSTRACT A survey of gastrointestinal parasites of chickens was carried out in Akure, Ondo state, Nigeria between January and July, 2015. A total of three hundred and twenty seven (327) chickens of different breeds were examined for gastrointestinal infections. Faecal samples obtained from these chickens were prepared for microscopy using... Continue Reading
ABSTRACT This study was aimed at investigating the microbial load and the quality of indoor air Faith Mediplex Centre, Benin City, to ascertain their contribution to infection rate in the hospital. Air samples were assessed for three (3) months (June-August, 2015) using the settled plate methods. The study sites were divided into five (5) units;... Continue Reading
ABSTRACT An investigative study was carried out to determine the bacteriological qualities of borehole water samples in halls of residence in the University of Benin, Ugbowo Benin City. A total of five water samples were collected from the the various halls of residence in the school. Bacteriological analysis was carried out using standard... Continue Reading
CHAPTER ONE 1.0 INTRODUCTION Yoghurt is a fermented dairy product obtained from the lactic acid fermentation of milk. It is one of the most popular fermented milk products in the world and produced commercially at home. (Willey et al., 2008). In its commercial production, non fat or low fat milk is pasteurized cooled to 43°c and are... Continue Reading
CHAPTER ONE INTRODUCTION 1.1 Background of the Study The number of people with high blood pressure is in the increase and research has shown that this condition is heightened by overweight of the patient. That is to say, the more one increases in weight, the higher the tendency of him or her becoming hypertensive. Mori TA (2007). In essence, there... Continue Reading
ABSTRACT This study was conducted to determine the antibiotic resistance profile of Escherichial Coli isolate from apparently healthy domestic livestock viz: cow, goats and chicken from Osun State Nigeria. E. Coli was isolated using Eosin methylene Blue Agar (EMB) and identified by conventional microbiological technique. The isolate were tested... Continue Reading
CHAPTER ONE 1.0 INTRODUCTION Water is the one of the most common substances known and it is good solvent for many substances, water occur at room temperature as clear, colourless, tasteless liquid which freezing into ice at zero degree... Continue Reading
CHAPTER ONE 1.0 INTRODUCTION Gases, dust particles, water vapour and air contain microorganisms. There are vegetable cells and spores of bacteria, fungi and algae, viruses and protozoa cysts. Since air is often exposed to sunlight, it has a... Continue Reading
CHAPTER ONE 1.0 INTRODUCTION Yoghurt is a fermented milk product, produced with a yoghurt starter culture which is a mixed culture of streptococcus thermophilus and lactobacillus bulgaricus in a 1:1 ratio. S thermophilusenjoys a faster growth than L hulgricus. It adds flavours and aroma to the yoghurt, though both organisms in... Continue Reading
ABSTRACT This project work is aimed at examination of jollof rice served in hotels for pathogenic organisms and to find out possible pathogenic organisms in the food. Samples were collected from the hotels and were brought to the laboratory for analysis. The sample were... Continue Reading
CHAPTER ONE 1.0 INTRODUCTION A diverse microbial flora is associated with the skin and mucous membrane of every human being from shortly after birth until death. The human body which contains about 1013 cells routinely harbors about 1014... Continue Reading
CHAPTER 1.0 INTRODUCTION Fungi are a major group of living things, originally considered plants lacking chlorophyll, leaves, true stems, roots and reproducing by spores, but now treated as the separate kingdom fungi. They occur in all... Continue Reading
ABSTRACT Comparative analysis of water was carried out on well water, stream and pipe born water obviated within Emene locality of Enugu state the sample were subjected to some analytical tests final like presumptive tests, comfirmative test final like presumptive tests, comfirmative test, completed test, indole test and plate count for the... Continue Reading
ABSTRACT Urine Sample from pregnant women were analysed for bacteriuria. The result of irinalysis reveals the appearance –yellow and Cloudy, pH 6.0, protein-ve, Glucose-ve,Blood-ve, Urobilinogen. Normal, Ketone-ve, Nitrate tve, Bilirubin-ve, Ascobic acid –ve in some of the samples. The result of urine microscopy reveal pus cells. ... Continue Reading
ABSTRACT The prevalence of streptococcus pneumonia in pneumonia patients using university of Nigeria Teaching Hospital (UNTH) Enugu as a case study was carried out based on the fact of the pneumonia is one of the commonest afflictions of the aged people and children worldwide. A total of 50 samples were collected. 12 (24%) of the sample were from... Continue Reading
ABSTRACT Fungi associated with the spoilage of cocoyam ( Colocasia esculenta ) during storage at ambient temperature (28+20c) was investigated in this study. This was done by cutting the damaged portion of the cocoyam tubers and blend with diluents (water) to have even distribution of the microorganism and ensure ease of plating out. This done... Continue Reading
ABSTRACT A total of fifty faecal samples were collected form pupils in airport primary school Emene Enugu were used for the study. The duration of the investigation was between February August 2005. These samples were cultured and their causative organisms determined using macconkey agar, deocycholate citrate agar and an alkaline peptone water... Continue Reading
TABLE OF CONTENT Chapter one 1.0 Introduction Chapter Two 2.0 Literature review 2.1 History of canned tomato 2.2 Types of canned Tomatoes 2.3 Nutrition facts of canned tomatoes 2.4 Common... Continue Reading
ABSTRACT This work was done in order to investigate the microbial content of some bolted soft drivels sold in Enugu interlopes. Soft drink are consumed in large quantities by the different age groups, adult and children of different class of people all over the state. This is aided by the general availability of these drink almost every place... Continue Reading
ABSTRACT This project work is carried at the isolation and characterization of pathogenic bacteria from hawked suya meat, were bought in full from the sellers and was collected in raps of aluminum foil. It was then taken to the laboratory for analysis the samples were homogenized and serial dilution of the sample was made out using 5 ml sterile... Continue Reading
ABSTRACT In thus work the incidence of candidacies was studied using selected patients of university of Nigeria teaching hospital Enugu analyzed for the presence of candidacies yeast infection. The specimens were cultured on seaboards agar and blood agar and subculture isolated were C aibicans C Tropical C Knusei C Pseudoizo P cals C paraknusei... Continue Reading
ABSTRACT In order to access the prevalence and sensitivity pattern of urinary pathogens, 60 midstream urine samples from students of Caritas University were investigated using cultural methods. Samples were examined microscopically and cultured in blood agar and Macckonkey agar. Disk diffusion method was used for antibiotic testing. Of the 60... Continue Reading
ABSTRACT Juice was extracted from banana ( Musa sapientum) pulp with the addition of lemon juice and was inoculated with Baker’s yeast (Saccharomyces cerevisiae) and held at 30 for seven days. The result of the yeast count increases at 48hr, and at 96hr the yeast count decreased gradually. It ranges from 4.9x107 cfu/ml at 0hr,... Continue Reading
ABSTRACT Ten (10) samples of suya meat in Enugu were collected randomly and analyzed microbiologically and the isolates were identified as Staphylococcus aureus (35%) Escherichia coli (15%) Streptococcus species (15%) Pseudomanas (35%) . The most frequently isolated organism was Staphylococcus and Pseudomonas . The total viable... Continue Reading
HAPTER ONE 1.1 INTRODUCTION Canning food has been a labor of love for generations of families. Today has become an opportunity to take control of the food you and your family consume. The key to successful canning understands the acidity and spoilage factor of the food you wish to can, as well as the acceptable canning methods to process... Continue Reading
INTRODUCTION The primary function of intact skin is to control microbial population that live on the skin surface and to prevent underlying tissue from becoming colonized and invaded by potential pathogens (Ndip et. al., 2007). Exposure of subcutaneous tissue following a loss of skin integrity (i.e. wound) provides a moist, warm and nutritious... Continue Reading
ABSTRACT Yam popularly called “ Ji” in Igbo Language is a tropical crop belonging to the Family Dioscoreaceae in the genus Dioscorea . It has as many as 600 species out of which six are economically important staple species. These are: Dioscorea rotundata (white guinea yam), Dioscorea alata (water yam), Dioscorea bulbifera... Continue Reading
ABSTRACT Water is one of the indispensable resources for the continued existence of all living things including man. The provision of an adequate supply of safe drinking water was one of the eight components of primary health care identified by the International Conference on Primary Health care in 1978. This study investigated the bacteriological... Continue Reading
ABSTRACT Agriculture in the early days was generally considered too small an industry to have significant impact on the environment. The remarkable growth of the agriculture industry in many countries over the past decades has increased adverse impact on the environment. (Acketors, 2014). The cultivation of organisms in ponds (Tincker, 2012),... Continue Reading
ABSTRACT Dandruff is a scalp disorder which is characterized by excessive shedding of skin cells from the scalp. It is a common problem faced by people of all age groups. As the epidermal layer continually replaces itself, cells are pushed outward where they eventually die and flake off. For most individuals, these flakes of skin are too small... Continue Reading
IN KADUNA METROPOLIS, NIGERIA ABSTRACT There is high risk of contracting hepatitis B and C among individuals with psychotic disorders due to lifestyle factors and prisoners globally continue to demonstrate a higher prevalence of Hepatitis B and C... Continue Reading
ABSTRACT Microbial assessment of smoked Scombia scombia spp. of fish procured from two markets in Owerri metropolis was carried out. The samples were analyzed using bacteriological and fungi media. The average bacteria counts ranges from 3.1X106-6.8X106cfu/g while the average fungi counts ranges from 0.0-0.3X106cfu/g. Four species of bacteria... Continue Reading
ABSTRACT Probiotics are defined as live microorganisms that, when administered in adequate amounts, confer a health benefit on the host (FAO/WHO, 2001). Probiotics is a general name for microorganisms that are associated with beneficial effects for humans and animals. They contribute to intestinal microbial balance and play a role in maintaining... Continue Reading
ABSTRACT Fish is highly nutritious with high protein content. However, it is a suitable medium for growth of microorganisms, if poorly processed (Oparaku and Mgbenka, 2012). The growth of microorganisms and other non-microbial activities such as lipid oxidation contribute to the deterioration of fish products (Martin, 2010). An increase in the... Continue Reading
MICROORGANISM ASSOCIATED WITH SPOILAGE OF TOMATOES (CANNED TOMATOES) TABLE OF CONTENT Chapter one 1.0 Introduction Chapter Two 2.0 Literature review 2.1 History of canned tomato 2.2 Types of canned... Continue Reading
ABSTRACT Fungi associated with the spoilage of cocoyam ( Colocasia esculenta ) during storage at ambient temperature (28+20c) was investigated in this study. This was done by cutting the damaged portion of the cocoyam tubers and blend with diluents (water) to have even distribution of the microorganism and ensure ease of plating out. This done... Continue Reading
ABSTRACT This work is aimed at determining the incidence of bacterial injections such as Salmonella and Escherichia coli which cause health hazards to the poultry in their feeds. Sample feed were obtained from commercial mash operation/ pellet mill operation at New heaven and top feed at Ogbete main market Enugu. The... Continue Reading
ABSTRACT The researcher investigated the effects of maternal nutrition on the exclusive breastfeeding of infants in Egor Local Government Area of Edo State. The purpose of the study was to find out the effects of maternal nutrition, socio-economic status, occupation, and perception on the exclusive breastfeeding of infants in Egor Local... Continue Reading
ABSTRACT Cosmetic(creams) need not be sterile,however they must not be unduly contaminated with micro-organism and should remain in a stable state throughout the shelf life of the product (or when be used by the consumer).the aim of this project was to determine the microbial load in selected creams and to identify the specific contaminants. For... Continue Reading
ABSTRACT Two medicated soaps and one local soap were investigated for their antibacterial activities against Staphylococcus aureus . Identification of the bacteria species was by standard microbiological techniques which included colonial examination, gram staining, and biochemical testing. Minimum inhibitory and minimum bactericidal activities... Continue Reading
Abstract The emergence of multiple antibiotic resistance in bacteria and the indiscriminate use of antibiotics contribute to the dissemination of resistant pathogens in the environment which may cause problems in therapy and is a serious public health issue. This study was conducted to determine the incidence of Pseudomonas aeruginosa and ... Continue Reading
ABSTRACT Crude oil and kerosene have useful applications in the society and are, therefore, used as sources of income and energy. However, the spills arising from crude oil and kerosene in our environment are becoming a visible problem in our ecosystem. Crude oil and kerosene pollute our farmlands and other arable lands and may negatively affect... Continue Reading
ABSTRACT Honey has been used traditionally for ages to treat infectious diseases. Antimicrobial activity of honey is complex due to the involvement of multiple bioactive compounds. The physico-chemical and antimicrobial properties of honey varieties from Apis mellifera and stingless bees, Hypotrigona sp. (Okotobo - Igbo) and Melipona ... Continue Reading
ABSTRACT Comparative examination of three main sources of water supply in Independence layout Enugu was carried out with a view to determine their levels of contamination of bacteria. The sources of water examined are tap water, well water and stream water. The pour plate method was used to examine the water samples. Bacteria isolated from the... Continue Reading
ABSTRACT In order to access the prevalence and sensitivity pattern of urinary pathogens, 60 midstream urine samples from students of Caritas University were investigated using cultural methods. Samples were examined microscopically and cultured in blood agar and Macckonkey agar. Disk diffusion method was used for antibiotic testing. Of the 60... Continue Reading
ISOLATION OF MICROCOCCUS FROM FERMENTED UGBA TABLE OF CONTENT Title page Certification Dedication Acknowledgement Table of content CHAPTER ONE 1.0 Introduction 1.1 Aims and objectives CHAPTER TWO Literature review 2.1 Fermented foods 2.2 Pentaclethra macrophylla 2.3 Botany 2.4 Ecology 2.5 Growt and... Continue Reading
CHAPTER ONE 1.0 INTRODUCTION Yoghurt is a cultural diary product produced by lactic fermentation of milk (Hui, 1992). Yoghurt is produced by the controlled fermentation of milk by two species of bacteria; Lactobacillus bulgaricus and Strptococcus thermophilus . These two species of bacteriae have now... Continue Reading
CHAPTER ONE 1.0 INTRODUCTION Ugba also called ukpaka is a popular food delicacy in Nigeria especially among Ibo ethnic group. It is rich in protein and is obtained by a solid state fermentation of the seed of African oil bean tree (Pentaclethra macrophylla Benth). It is essential food item from various traditional ceremonies... Continue Reading
ABSTRACT Ethanol, Bleach and Phenolics are three kinds of disinfectants which have been widely used in common laboratories. In this study, a compared experiment on these three disinfectants efficiency was conducted against Staphylococcus aureus and Pseudomonas aeruginosa using agar hole diffusion method. Different concentrations of bleach... Continue Reading
CHAPTER ONE 1.0 INTRODUCTION Yoghurt is a fermented dairy product obtained from the lactic acid fermentation of milk. It is one of the most popular fermented milk products in the world and produced commercially at home. (Willey et al., 2008). In its commercial production, non fat or low fat milk is pasteurized cooled to 43°c and are... Continue Reading
ABSTRACT Comparative analysis of water was carried out on well water, stream and pipe born water obviated within Emene locality of Enugu state the sample were subjected to some analytical tests final like presumptive tests, comfirmative test final like presumptive tests, comfirmative test, completed test, indole test and plate count for the... Continue Reading
INTRODUCTION 1.1 Background of the study Poultry are collection of birds raised commercially or domestically for meat, egg and feathers. Chickens, ducks turkeys and geese are of primary importance while guinea fowl and squabs (pigeons) are chiefly of local interest. These birds are source of income and food to the... Continue Reading
CHAPTER ONE 2.0 INTRODUCTION The term poultry used in agriculture generally refers to all domesticated birds kept for egg or meat production. These includes chickens (domestic fowls), Grallus domestics, turkeys, ducks and geese. This project will deal largely with the chicken which are the most common domestic fowl. The... Continue Reading
ABSTRACT The study for the production of protease from Aspergillus flavus using wheat bran as substrate under Solid State Fermentation was conducted in University of Abuja, Department of Microbiology. Aspergillus flavus was isolated from spoilt bread and was identified on the basis of the morphological assessment such as macroscopic and... Continue Reading
Abstract The study was carried out with aim to isolate Staphylococcus aureus from cow meat and determine the antibiogram pattern of S. aureus . Three samples of cow meat from three different locations (Gwagwalada market, Abattoir market and Kasuan Dare ) in Gwagwalada were collected to isolate S. aureus from the samples. The organisms... Continue Reading
ABSTRACT The study examined the various fungal pathogens associated with tomatoes spoilage as well as their preliminary taxonomic grouping up to genus level. 25 samples of the spoiled tomatoes were collected from five locations within Gwagwalada area council. Locations include: Passo Village, Gwagwalada market, Phase III, New Kutunku and Dagiri.... Continue Reading
ABSTRACT In comparing the antimicrobial strength of three most common antibiotics, which includes: streptomycin chloranphenicol and gentarccin. Samples of urine and high vaginal swab (H.V.S) were collected from park lane Enugu. The organisms isolated were pure culture of staphylococcus aureus and Escherichia coli Sensitivity test was carried out... Continue Reading
ABSTRACT Twenty samples of frozen chicken from five different sellers were analyzed to determine their bacteriological load; the samples were collected from Gwagwalada market. All the frozen chicken samples from the five sellers examined were contaminated with some bacterial... Continue Reading
ABSTRACT An investigative study was carried out to determine the bacteriological qualities of borehole water samples in halls of residence in the University of Benin, Ugbowo Benin City. A total of five water samples were collected from the the various halls of residence in the school. Bacteriological analysis was carried out using standard... Continue Reading
ABSTRACT Nunu is Nigerian locally fermented milk product commonly prepared by Hausa/Fulani cattle rearers. It’s mostly available in the Northern part of Nigeria. It’s production and consumption derives food security and economic benefits to the rural people in the region. However, the process characteristics results in products which are not... Continue Reading
CHAPTER ONE INTRODUCTION Chlamydiae are small gram-negative obligate intracellular microorganisms that preferentially infect squamocolumnar epithelial cell (www. Google. Com.2010). Chlamydia is the most prevalent sexually transmitted disease resulting in urethritis, with frequencies exceeding those of Neisseria gonorrhoeae ... Continue Reading
CHAPTER ONE 1.0 INTRODUCTION Ugba also called ukpaka is a popular food delicacy in Nigeria especially among Ibo ethnic group. It is rich in protein and is obtained by a solid state fermentation of the seed of African oil bean tree (Pentaclethra macrophyllaBenth). It is essential food item from various traditional ceremonies... Continue Reading
ABSTRACT The study is aimed at evaluating the microbiological quality control of soymilk beverage sold in I.M.T campus 3 Enugu. The soymilk samples were collected randomly from all the soymilk hawkers in campus 3 I.M.T Enugu. 30 soymilk samples were collected and used for the study. All the sample were freshly prepared, serial dilution of the... Continue Reading
CHAPTER ONE 1.0 INTRODUCTION Ugba also called ukpaka is a popular food delicacy in Nigeria especially among Ibo ethnic group. It is rich in protein and is obtained by a solid state fermentation of the seed of African oil bean tree (Pentaclethra macrophyllaBenth). It is essential food item from various traditional ceremonies... Continue Reading
CHAPTER ONE 1.0 Introduction 1.1 Background of the Study The number of people with high blood pressure is in the increase and research has shown that this condition is heightened by overweight of the patient. That is to say, the more one increases in weight, the higher the tendency of him or her becoming... Continue Reading
ABSTRACT Microbial evaluation of twenty samples of raw milk from a diary farm (Emene fulani cattle rearers) was carried out using five method: viz direct microcopies count nutrient agar count, Blood agar count, Mac conkey agar count (celiforms only) and Acid fast bacilli staring was done to assay for the presence of the Tubercle bacillus. The... Continue Reading
CHAPTER ONE 1.0 INTRODUCTION Yoghurt is a fermented dairy product obtained from the lactic acid fermentation of milk. It is one of the most popular fermented milk products in the world and produced commercially at home. (Willey et al., 2008). In its commercial production, non fat or low fat milk is pasteurized cooled to 43°c and are... Continue Reading
INTRODUCTION 1.1 Background of the study Antiseptics and disinfectants are used extensively in hospitals and other health care settings for a variety of topical and hard-surface applications. In particular, they are an essential part of infection control practices and aid in the prevention of nosocomial infections. Mounting concerns over the... Continue Reading
ABSTRACT Normal vaginal flora contains a wide range of microorganisms. Bacterial vaginosis BV is the main reason of vaginal discharge. Many gram positive and gram negative rods i.e. E.coli, Klebsiella, Proteus, Acinetobacter and Pseudomonas spp. are major... Continue Reading
THESTUDY ON THE EFFECT OF GARLIC AND HONEY ON SOME UPPER RESPIRATORY TRACT INFECTIONS TABLE OF CONTENT CHAPTER ONE MEDICINAL EFFECT OF HONEY- 1.2 MEDICINAL EFFECT OF GARLIC 1.3 UPPER RESPIRATORY TRACT INFECTIONS BROBLEMS CHPATER TWO 2.0 LITERATURE REVIEW - - 2.1 HISTORY OF GARLIC AND HONEY - - - 2.2 WHAT IS UPPER RESPIRATORY INFECTION? 2.3 CAUSES... Continue Reading
ABSTRACT A study was conducted to determine the presence of Salmonella species in Frozen Foods in Owerri in August, 2000. A total of 30 Food samples were collected from the different market in ten different locations in Owerri and examined for the presence of... Continue Reading
CHAPTER ONE INTRODUCTION 1.0 INTRODUCTION Radioactivity can be defined as the spontaneous disintegration of nucleus of an atom with an emission of alpha, beta particles and gamma ray. The radiation burden of the... Continue Reading
CHAPTER ONE 1.0 INTRODUCTION Yoghurt is a fermented dairy product obtained from the lactic acid fermentation of milk. It is one of the most popular fermented milk products in the world and produced commercially at home. (Willey et al., 2008). In its commercial production, non fat or low fat milk is pasteurized... Continue Reading
ABSTRACT Comparative analysis of water was carried out on well water, stream and pipe born water obviated within Emene locality of Enugu state the sample were subjected to some analytical tests final like presumptive tests, comfirmative test... Continue Reading
CHAPTER ONE 1.1 INTRODUCTION Water is of the materials require to sustain life. It is naturally, available and covers 70% of th earth’s crust. With the increasing demand of clean, potable water for domestic and industrial use, developing, locating and maintaining a cost-effective water treatment techniques is... Continue Reading
ABSTRACT Onion is associated with micro-organisms which are capable of causing spoilage. Onions with rots were examined microscopically. A solution of it was made and the serial dilution of 1:10-1¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬ to 1:1-5 were made from 10ml of the onions... Continue Reading
ABSTRACT The survey of urinary sehistomiass in Ishele in Amagunze, Nkanu East local Government was carried out using two methods of diagnosis i.e combination of questionnaire on clinical signs and symptoms, and laboratory... Continue Reading
. ABSTRACT Microbial examination of spoilt Avocado fruits was carried out to obtained the causal organism. A total of 60 avocado from Ogbete Main market and New market Enugu were examined by culturing on nutrient agar and sabourand dextrose agar (SM), for bacteria and fungi causing post – harvest... Continue Reading
ABSTRACT Starch was extracted from two varieties of sorghum bicolor grains (white and red varieties) steeped for 6, 8 and 12 hours by wet milling method, the starch samples were analysed for yield, functional... Continue Reading
ABSTRACT Samples of water from the different hostels in campus II and other source of water production namely Ajali-owa water, from bore hole 9th mile and spring water from GRA water board were water collecterd and analysed using... Continue Reading
ABATRACT Microbial evaluation of twenty samples of raw milk from a diary farm (Emene fulani cattle rearers) was carried out using five method: viz direct microcopies count nutrient agar count, Blood agar count, Mac conkey agar count (celiforms only) and Acid fast bacilli staring was done to assay... Continue Reading
ABSTRACT Biscuit was produced from malted sorghum (Sorghum bicolor) and wheat flour blend. Sorghum grains were sorted, steeped in water, germinated and kilned. Four samples of biscuit were produced using sorghum and wheat in the following ratios samples A; 30: 70,... Continue Reading
ABSTRACT This project work is aimed at examination of jollof rice served in hotels for pathogenic organisms and to find out possible pathogenic organisms in the food. Samples were collected from the hotels and were brought to the laboratory for analysis. The sample were... Continue Reading
ABSTRACT Urine Sample from pregnant women were analysed for bacteriuria. The result of irinalysis reveals the appearance –yellow and Cloudy, pH 6.0, protein-ve, Glucose-ve,Blood-ve, Urobilinogen. Normal, Ketone-ve, Nitrate tve, Bilirubin-ve,... Continue Reading
THE PEEVALENCE OF STREPTOCOCCUS PNEUMONIAE IN PNEUMONIA PTIENTS IN ENUGU METROPOLI A CASE STUDY OF UNTH ENUGU. ABSTRACT The prevalence of streptococcus pneumonia in pneumonia patients using university of Nigeria Teaching Hospital (UNTH) Enugu as a case study was carried out based on the fact of the pneumonia is one of the commonest afflictions of... Continue Reading
ABSTRACT This work is aimed at determining the incidence of bacterial injections such as Salmonella and Escherichia coli which cause health hazards to the poultry in their feeds. Sample feed were obtained from commercial mash operation/ pellet mill operation at New heaven... Continue Reading
ABSTRACT In the work, samples of Garri obtained from Ogbete market were evaluated for microbial contamination. Four sample of Garri from different sources of supple were collected from Ogbete main market Enugu Okwo, Nkalagu, Emene, and Abakaliki respectively. The sample were extracted... Continue Reading
PROBLEMS AND PROSPECTS OF COMMUNITY BANKS IN THE RURAL DEVELOPMENT OF NIGERIA. (A CASE STUDY OF OGUI COMMUNITY BANK, ENUGU SOUTH L. G. A.) ABSTRACT The community banking scheme has been in operation since December 1990, and has to a large extent achieved its objectives as a vehicle for poverty alleviation at the grassroots’ level. With about... Continue Reading
TABLE OF CONTENTS CHAPTER ONE 1.0 Introduction CHAPTER TWO 2.0 Causes and Effect of Malnutrition 2.1 Under Malnutrition 2.2 Malnutrition in Children 2.3 Psychological Effect 2.4 Food Habits 2.5 Taboos and Superstition 2.6 Nutritional Basic Education CHAPTER THREE 3.0 Obesity 3.1 Causes Effect of... Continue Reading
ABSTRACT Fungi associated with the spoilage of cocoyam (Colocasia esculenta) during storage at ambient temperature (28+20c) was investigated in this study. This was done by cutting the damaged portion of the cocoyam tubers and blend with diluents (water) to have even... Continue Reading
GASTROENTERITIS IN PRIMAY SCHOOL CHILDERN IN ENUGU METROPOLIS (6 – 12 YRS) ABSTRACT A total of fifty faecal samples were collected form pupils in airport primary school Emene Enugu were used for the study. The duration of the investigation was between February August 2005. These samples were cultured and their causative organisms determined... Continue Reading
ABSTRACT Fungi associated with the spoilage of cocoyam (Colocasia esculenta) during storage at ambient temperature (28+20c) was investigated in this study. This was done by cutting the damaged portion of the cocoyam tubers and blend with diluents (water) to have even... Continue Reading
ABSTRACT The isolation and identification of bacteria associated with 150ml sepsis studies were carried out to 250 patient at National Orthopedic Hospital Enugu. (250) Two hundred and fifty patients with 150ml sepstis was grouped in to in patients and out patients. The sample... Continue Reading
ANAEMIA IN PREGNANCY (A CASE STUDY OF PREGNANT WOMEN ATTENDING ANTENATAL CLINIC AT UNIVERISTY OF NIGERIA TEACHING HOSPITAL. (UNTH, ENUGU) ABSTRACT The study on anaemia in pregnancy is aimed at determining packed cell volume (PCV), Haemoglobin (Hb) level and Erythrocyte sedimentation rate (ESR) of pregnant women, as well as the selferity,... Continue Reading
TABLE OF CONTENTS CHAPTER ONE 1.0 Introduction 1.1 Statement of the problem 1.2 Aims/Objective of the Study 1.3 Limitation of the Study 1.4 Significance of the Study CHAPTER TWO 2.0 Literature Review 2.1 Maize as Sweet Corn 2.2 Types of Sugary Corn and Their... Continue Reading
ISOLATIOIN AND CHARACTERISATION OF BACTERIA ASSOCIATED WITH HAWKED SUYA- MEAT (CASE STUDY OF ENUGU). ABSTRACT This project work is carried at the isolation and characterization of pathogenic bacteria from hawked suya meat, were bought in full from the sellers and was collected in raps of aluminum foil. It was then taken to the laboratory for... Continue Reading
THE PREVALENCE OF TRICHOMONA VAGINALIS AMONG ADULTS IN “OSUMENYI” IN NNEWI SOUTH LOCAL GOVERNMENT AREA ANAMBRA STATE ABSTRACT The prevalence of Trichomonas Vaginalis among adult in “Osumenyi” in Nnewi south local government Area of Anambra state was carried out. This was done using a stotal of one hundred adults from there different... Continue Reading
ABSTRACT This research work was on maize (Zea may) abject in sorghum vulgare) beer brewing the sorghum was malted and milled. Malting was achieved by sleeping the grains in water at 280c for 48 germination of the grain was allowed for 72 hrs and kilmed in air oven at 550c for 48 hrs. maize as an adjuct was in... Continue Reading
ABSTRACT Comparative analysis of water was carried out on well water, stream and pipe born water obviated within Emene locality of Enugu state the sample were subjected to some analytical tests final like presumptive tests, comfirmative test... Continue Reading
ABSTRACT Invitro determination of bacteriocidal effect of garlic extract on staphylococcus aureus causing skin and urinary tract infection (UTI) on some female patients were studied. High vagina swab (HVS) samples were collected randomly from selected number of... Continue Reading
ABSTRACT In thus work the incidence of candidacies was studied using selected patients of university of Nigeria teaching hospital Enugu analyzed for the presence of candidacies yeast infection. The specimens were cultured on seaboards agar... Continue Reading
ABSTRACT In comparing the antimicrobial strength of three most common antibiotics, which includes: streptomycin chloranphenicol and gentarccin. Samples of urine and high vaginal swab (H.V.S) were collected from park lane Enugu. The organisms isolated were... Continue Reading
CHAPTER ONE 1.0 INTRODUCTION Many of the developing countries including African countries like Nigeria practice traditional medicine as its main source of health care. This traditional medicine is normally gotten from plant origin [Rehan Ahmad et al, 2008,... Continue Reading
MICROORGANISM ASSOCIATED WITH SPOILAGE OF TOMATOES (CANNED TOMATOES) TABLE OF CONTENT Chapter one 1.0 Introduction Chapter Two 2.0 Literature review 2.1 History of canned tomato 2.2 Types of canned Tomatoes 2.3 Nutrition facts of canned tomatoes 2.4 Common additions of canned tomato 2.5 causes of canned tomatoes spoilage 2.6 Control/preservation... Continue Reading